Anti-Mouse gp130/IL6ST Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 mouse splenocytes using 98275-1-RR

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse gp130/IL6ST Rabbit Recombinant Antibody (98275-1-RR, Clone: 242236B6) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed.

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  FC experiment of C57BL/6 mouse splenocytes using 98275-1-RR

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse gp130/IL6ST Rabbit Recombinant Antibody (98275-1-RR, Clone: 242236B6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98275-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98275-1-RR against Mouse gp130/IL6ST were performed. The affinity constant is below 1 pM.

gp130/IL6ST Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98275-1-RR

Clone No.242236B6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

IL-6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130, CD130

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Tested Applications

• Positive FC detected in: C57BL/6 mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98275-1-RR targets gp130/IL6ST in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 6 signal transducer
• Calculated Molecular Weight: 102kDa, 917aa
• GenBank Accession Number: NM_010560.3
• Gene Symbol: Il6st
• Gene ID (NCBI): 16195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q00560
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). The binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).