- Featured Product
- KD/KO Validated
hamartin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 20988-1-AP
Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein
|Positive WB detected in||HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
20988-1-AP targets hamartin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||tuberous sclerosis 1|
|Calculated molecular weight||130 kDa|
|Observed molecular weight||70 kDa, 130-140 kDa|
|GenBank accession number||NM_000368|
|Gene ID (NCBI)||7248|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TSC1, also named as KIAA0243 and TSC, is implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. In complex with TSC2, TSC1 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It seems not to be required for TSC2 GAP activity towards RHEB. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Defects in TSC1 are the cause of tuberous sclerosis complex (TSC). Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC). The antibody is specific to TSC1. This antibody can recognize two isoforms: 70 kDa (667aa) and 130 kDa.
|Product Specific Protocols|
|WB protocol for hamartin antibody 20988-1-AP||Download protocol|
|IHC protocol for hamartin antibody 20988-1-AP||Download protocol|
|IF protocol for hamartin antibody 20988-1-AP||Download protocol|
|IP protocol for hamartin antibody 20988-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Tuberous sclerosis complex exhibits a new renal cystogenic mechanism.
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Activation of mTORC1 in fibroblasts accelerates wound healing and induces fibrosis in mice.
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