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Recombinant Human CD4 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0333

Validation Data Gallery

  • Purity of Recombinant Human CD4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD4 protein Lys26-Pro396 (Accession# P01730) with a His tag at the C-terminus.
GeneID 920
Accession P01730
PredictedSize 42.4 kDa
SDS-PAGE 50-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD4 is a transmembrane glycoproteinof the immunoglobulin super-family (IgSF), containing four extracellular Ig-like domains, ahydrophobic transmembrane region and a 40-residue cytoplasmictail. CD4 is expressed T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells.CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interactionand it is involved in T cell differentiation, development and activation by interacting with antigen-presenting cells (APCs). The cytoplasmic tail of CD4 associates with the Lck kinase, which in turn activates the signaling components of the T cell receptor (TCR)-CD3 complexes.CD4 also serves as a receptor for human immunodeficiency virus (HIV) by binding to the viral envelope glycoprotein gp120.

References:

1.Tseng WY, et al. (2013). PLoS One. 8(5):e63963.
2.Bowers K, et al. (1997). Int J Biochem Cell Biol. 29(6):871-875.
3.Graziani-Bowering G, et al. (2002). Exp Cell Res. 279(1):141-152.
4.Moore JP, et al. (1997). Curr Opin Immunol. 9: 551–562.