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Recombinant Human OX40/CD134 protein (Myc Tag, His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 0.3-1.2 ng/mL

Cat no : Eg0022

Validation Data Gallery

  • Purity of Recombinant Human OX40 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human OX40 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human OX40 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human OX40L (hFc tag) with a linear range of 0.3-1.2 ng/mL.

    Immobilized Human OX40 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human OX40L (hFc tag) with a linear range of 0.3-1.2 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human OX40 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human OX40L (hFc tag) with a linear range of 0.3-1.2 ng/mL.
Expression HEK293-derived Human OX40 protein Leu29-Ala214 (Accession# P43489) with a Myc tag and a His tag at the C-terminus.
GeneID 7293
Accession P43489
PredictedSize 22.5 kDa
SDS-PAGE 38-50 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD134 (OX40, TNFRSF4) is a member of the TNFR-superfamily of receptors. Recent evidence indicates that OX40-OX40L coupling is critical for cell survival and proliferation. While OX40 is expressed predominantly on T-lymphocytes early after antigen activation, OX40L is expressed on activated antigen presenting cells and endothelial cells within acute inflammatory environments. The tumour necrosis factor receptor OX40 (CD134) is activated by its cognate ligand OX40L (CD134L, CD252) and functions as a T cell co-stimulatory molecule. OX40 is expressed on activated T cells, and in the mouse at rest on regulatory T cells (Treg). CD134 can interact with TRAF2, TRAF3, and TRAF5. OX40 is an immune checkpoint in cancer and its presence in cancer is a good prognosis, making it a highly relevant target for the development of new immunotherapies.

References:

1.Wu LY. et al. (2020). Exp Neurol. 326:113179.
2.Gough MJ. et al. (2009). Adv Exp Med Biol. 647:94-107.
3.Webb GJ. et al. (2016). Clin Rev Allergy Immunol. 50(3):312-32. 
4.Cebada J. et al. (2021). Expert Opin Ther Pat. 31(1):81-90.