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Recombinant Human ST2/IL-1RL1 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 47-188 ng/mL

Cat no : Eg0116

Validation Data Gallery

  • Purity of Recombinant Human ST2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ST2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human IL-33 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human ST2 (Myc tag, His tag) with a linear range of 47-188 ng/mL.

    Immobilized Human IL-33 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human ST2 (Myc tag, His tag) with a linear range of 47-188 ng/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human IL-33 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human ST2 (Myc tag, His tag) with a linear range of 47-188 ng/mL.
Expression HEK293-derived Human ST2 protein Lys19-Phe328 (Accession# Q01638-2) with a Myc tag and a His tag at the C-terminus.
GeneID 9173
Accession Q01638-2
PredictedSize 37.5 kDa
SDS-PAGE 50-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Protein ST2, also known as IL1RL1, is a member of the interleukin 1 receptor family. The gene of ST2 encodes three splice variants: a soluble secreted form (sST2), a transmembrane receptor form (ST2L), and a variant form of ST2 (ST2V). Interleukin-33 (IL-33) has been identified as a functional ligand of ST2L. IL-33 exerts its cellular functions by binding a receptor complex composed of ST2L and IL-1R accessory protein (IL-1RAcP). The interaction of IL-33 and ST2L activates mitogen-activated protein kinases and several biochemical pathways. The end of these reactions is the activation of the inhibitor of nuclear factor-kB (NF-kB) kinase complex, triggering NF-kB activity. sST2 seems to act as a decoy-receptor for IL-33: it binds IL-33 thus subtracting such a molecule from the interaction with ST2L. sST2 is formed by many cells and sST2 level is increased as response to myocardial stress, as well as in inflammatory conditions, including allergic asthma.

References:

1. Kuroiwa K, et al. (2000) Hybridoma.19(2):151-9.
2. Pecaric-Petkovic T, et al. (2009) Blood. 113(7):1526-34.
3. Ciccone MM, et al. (2013) Molecules. 18(12):15314-28.
4. Pascual-Figal DA, et al. (2015) Am J Cardiol. 115(7 Suppl):3B-7B.