ChromoTek iST Myc-Trap Kit for AP-MS sample preparation of Myc-fusion proteins

Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)

Specificity

Myc-Tag

Applications

IP, MS

Conjugate

Agarose

Cat no : ytak-iST

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)

ApplicationsIP, MS
Clonality VHH
HostAlpaca
Affinity1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Specificity/TargetMyc-tag sequence motif EQKLISEEDL. Endogenous c-myc is NOT bound.
Coupled VHHRecombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 7 µg of recombinant Myc-tagged MBP
Storage Buffer20% ethanol
Storage ConditionKit is shipped at ambient temperature. Upon receipt store Myc-Trap at 4°C and Digest at -20°C.

Kit components

ComponentDescription
Myc-Trap Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.