ChromoTek mNeonGreen-Trap Agarose

Validation Data Gallery

• 

  Immunoprecipitation of mNeonGreen:Coomassie and Western Blot; Western blot indicates high effectiveness of pull-down.I: Input, FT: Flow-through, B: Bound.

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

Cat No. nta

Publications(11)

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag

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Product Information

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

• Description: Immunoprecipitation of mNeonGreen-fusion proteins and their interacting factors with anti-mNeonGreen Nanobody conjugated to beads.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

  • Short incubation time of about 60 minutes

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: mNeonGreen; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 30 μg of recombinant mNeonGreen per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 2 nM
• Compatibility with mass spectrometry: The mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827593
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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Publications

Application	Title
IP	Cell Stem Cell; Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit.; Authors - Christopher S Morrow
CoIP	Dev Cell; The ER ladder is a unique morphological feature of developing mammalian axons.; Authors - Emiliano Zamponi
IP	Proc Natl Acad Sci U S A; The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export.; Authors - Peiwei Liu
IP	J Cell Biol; SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility.; Authors - Sahezeel Awadia
IP	J Cell Biol; The HSPG syndecan is a core organizer of cholinergic synapses.; Authors - Xin Zhou
IP	Plant J; Elucidation of the late steps in the glycan-dependent ERAD of soluble misfolded glycoproteins; Authors - Jennifer Schoberer