ChromoTek mNeonGreen-Trap Agarose beads

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

Ready to use reagent

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Publications

3

Cat no : nta

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag

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Validation Data Gallery

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  Immunoprecipitation of mNeonGreen: Coomassie and Western Blot; Western blot indicates high effectiveness of pull-down. I: Input, FT: Flow-through, B: Bound.

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Product Information

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 2 nM
• Specificity/Target: mNeonGreen, Branchiostoma lanceolatum
• Coupled VHH: Recombinant, monoclonal anti-mNeonGreen single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Binding capacity: 10 µL slurry bind approximately 12 µg of recombinant mNeonGreen
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP-MS	Cell Stem Cell; Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit.; Authors - Christopher S Morrow
IP	J Cell Biol; SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility.; Authors - Sahezeel Awadia
IP	Proc Natl Acad Sci U S A; The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export.; Authors - Peiwei Liu