ChromoTek mNeonGreen-Trap Agarose beads
mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.
Ready to use reagent
Specificity
mNeonGreen
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : nta
Synonyms
Validation Data Gallery
Product Information
mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 2 nM |
Specificity/Target | mNeonGreen, Branchiostoma lanceolatum |
Coupled VHH | Recombinant, monoclonal anti-mNeonGreen single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Binding capacity | 10 µL slurry bind approximately 12 µg of recombinant mNeonGreen |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS mNeonGreen-Trap Agarose |
Split Fluorescent Protein Technology (PDF) |
Protocol |
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Protocol mNeonGreen-Trap Agarose (PDF) |
Brochure |
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Product brochure mNeonGreen (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |
Publications
Application | Title |
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Cell Stem Cell Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit. | |
J Cell Biol SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. | |
Proc Natl Acad Sci U S A The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export. |