• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Mouse SCF protein (His Tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 2-8 ng/mL

Cat no : Eg0402

Validation Data Gallery

  • Purity of Recombinant Mouse SCF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse SCF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.

    Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.
Expression HEK293-derived Mouse SCF protein Lys26-Ala189 (Accession# P20826-1) with a His tag at the C-terminus.
GeneID 17311
Accession P20826-1
PredictedSize 22.3 kDa
SDS-PAGE 25-35 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

SCF(Stem cell factor) also known as mast cell growth factorang kit ligand, is a hematopoietic growth factor. It plays an role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF is expressed in where hematopoiesis takes place, such as the fetal liver and bone marrow. SCF binding to c-kit ligand(CD117)causes the receptor to homodimerize and auto-phosphorylate at tyrosine residues. SCF and c-kit ligand are up-regulated in human malignancies including gastrointestinal stromal tumor, breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-kit pathway also plays a rolein the regulation of cell survival and proliferation, stem cell maintenance, mast cell development, migration.SCF and c-kit were confirmed as key role in hematopoietic function in adult mice.

References:

1.M Ogawa. et al.(1991)J Exp Med. 174(1): 63-71.
2.Gu Y. et al.(2011) PLoS One. 6(10): e25984.
3.Pedersen M. et al.(2008) Biochem Biophys Res Commun. 377(1): 98-103.
4.Talaiezadeh A. et al. (2012) Contemp Oncol (Pozn). 16(4): 306-9.
5.Bai CG. et al. (2012) World J Gastroenterol.18(23): 2929-37.
6.Lennartsson J. et al. (2012) Physiol Rev. 92(4): 1619-49.
7. Liang J. et al.(2013) Int J Biol Sci. 9(5): 435-43.