ChromoTek p53 N-term-Trap Agarose

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

Specificity

p53

Reactivity

Human

Applications

IP, CoIP

Type

Nanobody

Conjugate

Agarose

Publications

8

Cat no : pta

Synonyms

p53 N-term-Trap, p53 Trap, p53-Trap, Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

---

Validation Data Gallery

• 

  p53-N-term-Trap for immunoprecipitation of p53 isoform alpha, beta and gamma.I: Input, FT: Flow-through, B: Bound.

---

Product Information

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

• Description: Immunoprecipitation of p53 with p53-N-term-Trap Agarose.

  • Pull down of human p53 isoforms alpha, beta and gamma with p53-Trap-N-term

  • Immunoprecipitation of the human p53 proteome with complementary p53-C-term- and p53-N-term-Traps, e.g. p53 isoforms and variants

  • No heavy & light antibody chains in your downstream application

  • Short incubation time (60 min at +4 °C)

• Applications: IP, CoIP
• Specificity/Target: p53 (isoforms: alpha, beta, and gamma); epitope 1-81 aa
• Tested Reactivity: Human
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Compatibility with mass spectrometry: The p53-N-term-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631378
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Other Formats

p53 N term-Trap Agarose kit

Publications

Application	Title
IP	Cell Death Differ; The ubiquitin ligase LIN41/TRIM71 targets p53 to antagonize cell death and differentiation pathways during stem cell differentiation.; Authors - Duong Thi Thuy Nguyen
IP	Oncogene; Antagonizing CD105 enhances radiation sensitivity in prostate cancer.; Authors - Anisha Madhav
IP	PLoS Pathog; Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.; Authors - Dina Alzhanova
IP	Cancers (Basel); AML-Related NPM Mutations Drive p53 Delocalization into the Cytoplasm with Possible Impact on p53-Dependent Stress Response.; Authors - Aleš Holoubek
CoIP	J Virol; The ORF45 protein of Kaposi Sarcoma-associated Herpesvirus (KSHV) is an inhibitor of p53 signaling during viral reactivation.; Authors - Dina Alzhanova
IP	Sci Rep; Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways.; Authors - Karim Ullah