ChromoTek p53 N-term-Trap Agarose

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

Specificity

p53

Applications

IP, CoIP

Conjugate

Agarose

Publications

8

Cat no : pta

Synonyms

p53 N-term-Trap, p53 Trap, p53-Trap, Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Validation Data Gallery

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  p53-N-term-Trap for immunoprecipitation of p53 isoform alpha, beta and gamma.I: Input, FT: Flow-through, B: Bound.

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Product Information

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

• Applications: IP, CoIP
• Clonality: Monoclonal
• Host: Alpaca
• Specificity/Target: p53; Species-Reactivity: Tested on human
• Coupled VHH: Recombinant, monoclonal anti-p53 N-term single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Other Formats

p53 N term-Trap Agarose kit

Publications

Application	Title
IP	Biochem Biophys Res Commun; Phosphorylation of p53 by LRRK2 induces microglial tumor necrosis factor α-mediated neurotoxicity.; Authors - Dong Hwan Ho
IP	Chem Biol Interact; p53 mediated transcriptional regulation of long non-coding RNA by 1-hydroxy-1-norresistomycin triggers intrinsic apoptosis in adenocarcinoma lung cancer.; Authors - Vaikundamoorthy Ramalingam
IP	Sci Rep; Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways.; Authors - Karim Ullah
CoIP	J Virol; The ORF45 protein of Kaposi Sarcoma-associated Herpesvirus (KSHV) is an inhibitor of p53 signaling during viral reactivation.; Authors - Dina Alzhanova
IP	Cancers (Basel); AML-Related NPM Mutations Drive p53 Delocalization into the Cytoplasm with Possible Impact on p53-Dependent Stress Response.; Authors - Aleš Holoubek
IP	PLoS Pathog; Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.; Authors - Dina Alzhanova