ChromoTek p53 N-term-Trap Agarose
p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.
Cat no : pta
Synonyms
Validation Data Gallery
Product Information
p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.
| Applications | IP, CoIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Specificity/Target | p53 Species-Reactivity: Tested on human |
| Coupled VHH | Recombinant, monoclonal anti-p53 N-term single domain antibody (sdAb) fragment |
| Conjugate | Agarose |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| SDS |
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| p53-N-term-Trap Agarose SDS (PDF) |
| Protocol |
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| p53-N-term-Trap Agarose protocol (PDF) |
| Product flyer |
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| Mdm4- HdmX-Trap, p53-N-term-Trap & p53-C-term-Trap product flyer (PDF) |
Publications
| Application | Title |
|---|---|
Biochem Biophys Res Commun Phosphorylation of p53 by LRRK2 induces microglial tumor necrosis factor α-mediated neurotoxicity. | |
Chem Biol Interact p53 mediated transcriptional regulation of long non-coding RNA by 1-hydroxy-1-norresistomycin triggers intrinsic apoptosis in adenocarcinoma lung cancer. | |
Sci Rep Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways. | |
J Virol The ORF45 protein of Kaposi Sarcoma-associated Herpesvirus (KSHV) is an inhibitor of p53 signaling during viral reactivation. | |
Cancers (Basel) AML-Related NPM Mutations Drive p53 Delocalization into the Cytoplasm with Possible Impact on p53-Dependent Stress Response. | |
PLoS Pathog Novel modulators of p53-signaling encoded by unknown genes of emerging viruses. |