Phospho-TDP43 (Ser409/410) Polyclonal antibody

Phospho-TDP43 (Ser409/410) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 22309-1-AP


TARDBP, tdp 43, TDP43, tdp-43

Tested Applications

Positive WB detected inCalyculin A treated HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22309-1-AP targets Phospho-TDP43 (Ser409/410) in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-50 kDa, 25-35 kDa
GenBank Accession NumberNM_007375
Gene Symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.


Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 22309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Med

The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.

Authors - Wenzhang Wang

Nat Neurosci

TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.

Authors - Manling Xie

Mol Neurodegener

TDP-43-regulated cryptic RNAs accumulate in Alzheimer's disease brains

Authors - Virginia Estades Ayuso

JAMA Neurol

TDP-43 Accumulation Within Intramuscular Nerve Bundles of Patients With Amyotrophic Lateral Sclerosis.

Authors - Takashi Kurashige

Sci Adv

Calpain-2 as a therapeutic target in repeated concussion-induced neuropathy and behavioral impairment.

Authors - Yubin Wang

Acta Neuropathol

RNA aptamer reveals nuclear TDP-43 pathology is an early aggregation event that coincides with STMN-2 cryptic splicing and precedes clinical manifestation in ALS

Authors - Holly Spence


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


MANOHAR (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: hek293, neural stem cell

Shenyi (Verified Customer) (04-27-2022)

Staining with this antibody is very different from with the recombinant one (80007-1-RR) which I received as a free test vial. It showed strong signal in the nucleus while the recombinant one only showed signal in the cytoplasm. Now I'm confused: Could pTDP-43 (Ser409/410) also present in nucleus or it's the antibody being nonspecific?

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse brain slice
Phospho-TDP43 (Ser409/410) Antibody Immunofluorescence validation (1:500 dilution) in mouse brain slice (Cat no:22309-1-AP)

Azita (Verified Customer) (03-10-2020)

Immunofluorescent analysis of (4% PFA fixed) NSC-34 cells, using p- (109/110) TDP-43 antibody at 1/500 dilution and Alexa Flour conjugated Donkey anti Rabbit IgG (H+L) (Red). Bisbenzimide (Blue) labels cell nuclei.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: NSC-34 motor neuron like cell line
Phospho-TDP43 (Ser409/410) Antibody Immunofluorescence, validation (1/500 dilution) in NSC-34 motor neuron like cell line (Cat no:22309-1-AP)

Laura (Verified Customer) (01-10-2020)

Works well at this concentration on HEK293T lysate.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500

Owen (Verified Customer) (10-30-2019)

This antibody was used to image TDP43 in tissue and cell samples. The antibody was useful to image such a difficult target.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Human cortical tissue / motor neurons