|Positive WB detected in||Calyculin A treated HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
22309-1-AP targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||40-50 kDa, 25-35 kDa|
|GenBank accession number||NM_007375|
|Gene ID (NCBI)||23435|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
J Biol Chem
Aggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation.
Association between TDP-43 and mitochondria in inclusion body myositis.
J Alzheimers Dis
Phospho-Tau Changes in the Human CA1 During Alzheimer's Disease Progression.
Future Sci OA
Platelet phosphorylated TDP-43: an exploratory study for a peripheral surrogate biomarker development for Alzheimer's disease.
Acta Neuropathol Commun
Phosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis.
Acta Neuropathol Commun
Fragile X-associated tremor ataxia syndrome with co-occurrent progressive supranuclear palsy-like neuropathology.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Shenyi (Verified Customer) (04-27-2022)
Staining with this antibody is very different from with the recombinant one (80007-1-RR) which I received as a free test vial. It showed strong signal in the nucleus while the recombinant one only showed signal in the cytoplasm. Now I'm confused: Could pTDP-43 (Ser409/410) also present in nucleus or it's the antibody being nonspecific?
Azita (Verified Customer) (03-10-2020)
Immunofluorescent analysis of (4% PFA fixed) NSC-34 cells, using p- (109/110) TDP-43 antibody at 1/500 dilution and Alexa Flour conjugated Donkey anti Rabbit IgG (H+L) (Red). Bisbenzimide (Blue) labels cell nuclei.
Laura (Verified Customer) (01-10-2020)
Works well at this concentration on HEK293T lysate.
Owen (Verified Customer) (10-30-2019)
This antibody was used to image TDP43 in tissue and cell samples. The antibody was useful to image such a difficult target.