Tested Applications
| Positive WB detected in | Neuro-2a cells, Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22309-1-AP targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | TAR DNA binding protein |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | NM_007375 |
| Gene Symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_11182943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13148 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TDP43 (Ser409/410) antibody 22309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-TDP43 (Ser409/410) Polyclonal antibody (Cat# 22309-1-AP) has 157 citations published in journals including Nature Medicine, Cell, Nature Neuroscience, Nature Communications, Acta Neuropathologica, Brain, Neurobiology of Disease, and Journal of Neuropathology & Experimental Neurology. Associated research fields include ALS, frontotemporal dementia, Alzheimer's disease, neurodegeneration, traumatic brain injury, protein aggregation, and biomarker discovery.
| Species | Application | Title |
|---|---|---|
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Nat Nanotechnol Polystyrene nanoparticles trigger aberrant condensation of TDP-43 and amyotrophic lateral sclerosis-like symptoms | ||
Nat Aging LONRF2 is a protein quality control ubiquitin ligase whose deficiency causes late-onset neurological deficits | ||
JAMA Neurol TDP-43 Accumulation Within Intramuscular Nerve Bundles of Patients With Amyotrophic Lateral Sclerosis. | ||
Cell Stem Cell KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury |
Reviews
What customers say
Across 9 reviews, users rate this pTDP-43 antibody favorably for Western blotting, with consistent success at 1:1000 dilution across HEK293T, SH-SY5Y, and neural stem cells. Immunohistochemistry on Alzheimer's tissue yielded clear phospho-tangle staining with minimal nonspecific signal. However, immunofluorescence results are mixed—some users observed strong nuclear staining, raising questions about specificity compared to the recombinant counterpart (80007-1-RR). Overall, it is highly recommended for WB, while IF/IHC performance may vary by sample type and fixation protocol.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sonia (Verified Customer) (10-02-2025) | We detected increased phosphorylated TDP-43 (pTDP-43) in FTD iPSCs compared with WT, determined by immunoblotting cell lysates using pTDP-43 (Ser409/410). However, our immunolabeling data with pTDP-43 were unclear since it shows strong nuclear signal.
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Rashmi (Verified Customer) (09-25-2024) | Used for WB, Highly Recommended
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Vikas (Verified Customer) (07-11-2024) | Used for western blotting. Highly recommended.
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Madison (Verified Customer) (06-24-2024) | This antibody Stained well in the human tissue there were very clear phospho tangles and there was not a lot of nonspecific staining in the tissue.
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MANOHAR (Verified Customer) (03-06-2024) |
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Shenyi (Verified Customer) (04-27-2022) | Staining with this antibody is very different from with the recombinant one (80007-1-RR) which I received as a free test vial. It showed strong signal in the nucleus while the recombinant one only showed signal in the cytoplasm. Now I'm confused: Could pTDP-43 (Ser409/410) also present in nucleus or it's the antibody being nonspecific?
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Azita (Verified Customer) (03-10-2020) | Immunofluorescent analysis of (4% PFA fixed) NSC-34 cells, using p- (109/110) TDP-43 antibody at 1/500 dilution and Alexa Flour conjugated Donkey anti Rabbit IgG (H+L) (Red). Bisbenzimide (Blue) labels cell nuclei.
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Laura (Verified Customer) (01-10-2020) | Works well at this concentration on HEK293T lysate.
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Owen (Verified Customer) (10-30-2019) | This antibody was used to image TDP43 in tissue and cell samples. The antibody was useful to image such a difficult target.
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