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Recombinant Rat IL-2 protein (His Tag)

Species

Rat

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 5-19 ng/mL

Cat no : Eg0260

Validation Data Gallery

  • Purity of Recombinant Rat IL-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat IL-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Rat IL-2 (His tag) at 2 μg/mL (100 μL/well) can bind IL-2RA (rFc tag) with a linear range of 5-19 ng/mL.

    Immobilized Rat IL-2 (His tag) at 2 μg/mL (100 μL/well) can bind IL-2RA (rFc tag) with a linear range of 5-19 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Rat IL-2 (His tag) at 2 μg/mL (100 μL/well) can bind Rat IL-2RA (rFc tag) with a linear range of 5-19 ng/mL.
Expression HEK293-derived Rat IL-2 protein Met+Ala21-Gln155 (Accession# P17108) with a His tag at the C-terminus.
GeneID 116562
Accession P17108
PredictedSize 19.4 kDa
SDS-PAGE 19-26 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-2 (Interleukin-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells. IL-2 mediates its effects by binding to IL-2 receptors which are expressed by lymphocytes. IL-2 signal can be transduced by 3 different signaling pathways: JAK/STAT, PI3K/Akt/mTOR and MAPK/ERK pathway. IL-2 plays a role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. It also promotes proliferation of activated B cells and subsequently immunoglobulin production.

References:

1.M C Mingari. et al. (1984). Nature. 312(5995): 641-3.
2.Thomas R Malek. et al. (2010). Immunity. 33(2): 153-65.
3.Wei Liao. et al. (2011). Curr Opin Immunol. 23(5): 598-604.
4.Natalia Arenas Ramirez. et al. (2015). Trends Immunol. 36(12): 763-777.