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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human G-CSF protein

Activity

0.009-0.05 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1207

Synonyms

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin



Technical Specifications

GeneID 1440
Species human
Expression HEK293
Activity 0.009-0.05 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP09919
Molecular Mass 21 to 25 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Granulocyte colony-stimulating factor (G-CSF), also known as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils and trophoblast cells. G-CSF exerts its effects by binding to its receptor G-CSFR, in turn activating the JAK/STAT and Ras/Raf/MAP kinase pathways (PMID 9015216, 7509213, 9933603). G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy (PMID 7524753, 3264199). Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis (PMID 18524991).

Synonyms

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

Publications

SpeciesTitle
human

Breast Cancer Res Treat

A high-risk luminal A dominant breast cancer subtype with increased mobility.

Authors - Liping Guo
mouse

J Toxicol Environ Health A

The protective mechanisms underlying Ginsenoside Rg1 effects on rat sciatic nerve injury.

Authors - Dong-Sheng Huo
mouse

J Mol Cell Biol

Protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage.

Authors - Liming Hong
mouse

PLoS One

HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway.

Authors - NawalBen Haij
human

J Cell Mol Med

M2 macrophage-derived G-CSF promotes trophoblasts EMT, invasion and migration via activating PI3K/Akt/Erk1/2 pathway to mediate normal pregnancy.

Authors - Jinli Ding
human

J Virol

HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.

Authors - RémiPlanès