• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human G-CSF protein


0.009-0.05 ng/mL

Specific Activity

minimally 1.00 x 108 IU/mg

Species Reactivity




Cat no : HZ-1207


C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

Technical Specifications

GeneID 1440
Species Human
Expression HEK293
Activity(Primary) 0.009-0.05 ng/mL
Specific Activityminimally 1.00 x 108 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP09919
Molecular Mass 21 to 25 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.


Granulocyte colony-stimulating factor (G-CSF), also known as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils and trophoblast cells. G-CSF exerts its effects by binding to its receptor G-CSFR, in turn activating the JAK/STAT and Ras/Raf/MAP kinase pathways (PMID 9015216, 7509213, 9933603). G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy (PMID 7524753, 3264199). Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis (PMID 18524991).


C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin



Cell Stem Cell

Robo4 cooperates with CXCR4 to specify hematopoietic stem cell localization to bone marrow niches.

Authors - StephanieSmith-Berdan

J Mol Cell Biol

Protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage.

Authors - Liming Hong

Cell Prolif

Overproduced bone marrow neutrophils in collagen-induced arthritis are primed for NETosis: An ignored pathological cell involving inflammatory arthritis.

Authors - Danyi Xu

J Virol

HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.

Authors - RémiPlanès

J Cell Mol Med

M2 macrophage-derived G-CSF promotes trophoblasts EMT, invasion and migration via activating PI3K/Akt/Erk1/2 pathway to mediate normal pregnancy.

Authors - Jinli Ding

PLoS One

HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway.

Authors - NawalBen Haij