- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IFN gamma protein
Activity
0.02-0.14 ng/mL EC50
Specific Activity
2x10^7 IU/mg calibrated against IFN gamma reference standard (NIBSC cat no: 87/586). Reference use only. Specific activity is not routinely tested
Species Reactivity
human,mouse
Purity
>95%
Cat no : HZ-1301
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Validation Data Gallery
Technical Specifications
GeneID | 3458 |
Species | Human |
Expression | HEK293 |
Activity | 0.02-0.14 ng/mL EC50 |
Specific Activity | 2x10^7 IU/mg calibrated against IFN gamma reference standard (NIBSC cat no: 87/586). Reference use only. Specific activity is not routinely tested |
Purity | >95% |
Endotoxin | <1 EU/μg |
Accession Number | P01579 |
Molecular Mass | 29-32 kDa |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human albumin (HSA). |
Background
Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Publications
Species | Title |
---|---|
Pharm Biol β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice. | |
Cell Death Dis Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress. | |
ArXiv Darwin's Neural Network: AI-based Strategies for Rapid and Scalable Cell and Coronavirus Screening. | |
Pulm Pharmacol Ther Guanosine and uridine alleviate airway inflammation via inhibition of the MAPK and NF-κB signals in OVA-induced asthmatic mice. | |
PLoS One Mesenchymal stromal cell-derived extracellular vesicles reduce lung inflammation and damage in nonclinical acute lung injury: Implications for COVID-19. |