- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IFN gamma protein
Cat no : HZ-1301
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Validation Data Gallery
Technical Specifications
| GeneID | 3458 |
| Species | Human |
| Expression | HEK293 |
| Activity | 0.02-0.14 ng/mL EC50 |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P01579 |
| Molecular Mass | 29-32 kDa |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human albumin (HSA). |
Background
Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Publications
| Species | Title |
|---|---|
Pharm Biol β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice. | |
Cell Death Dis Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress. | |
ArXiv Darwin's Neural Network: AI-based Strategies for Rapid and Scalable Cell and Coronavirus Screening. | |
Pulm Pharmacol Ther Guanosine and uridine alleviate airway inflammation via inhibition of the MAPK and NF-κB signals in OVA-induced asthmatic mice. | |
PLoS One Mesenchymal stromal cell-derived extracellular vesicles reduce lung inflammation and damage in nonclinical acute lung injury: Implications for COVID-19. |
