HumanKine® recombinant human IFN gamma protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.02-0.14 ng/mL EC50

Specific Activity

2x10^7 IU/mg calibrated against IFN gamma reference standard (NIBSC cat no: 87/586). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Publications

11

Cat no : HZ-1301

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

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Validation Data Gallery

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  The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  Recombinant human IFN gamma (HZ-1301) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

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Technical Specifications

• GeneID: 3458
• Species: Human
• Expression: HEK293
• Activity: 0.02-0.14 ng/mL EC50
• Specific Activity: 2x10^7 IU/mg calibrated against IFN gamma reference standard (NIBSC cat no: 87/586). Reference use only. Specific activity is not routinely tested
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P01579
• Molecular Mass: 29-32 kDa
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human albumin (HSA).

Background

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

Publications

Species	Title
mouse	Pharm Biol; β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice.; Authors - Rui Liu
mouse	Cell Death Dis; Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress.; Authors - Yuexin Tian
human,mouse	ArXiv; Darwin's Neural Network: AI-based Strategies for Rapid and Scalable Cell and Coronavirus Screening.; Authors - Sang Won Lee
human	Cell Rep; Critical role of Syk-dependent STAT1 activation in innate antiviral immunity.; Authors - Shasha Liu
human	Pulm Pharmacol Ther; Guanosine and uridine alleviate airway inflammation via inhibition of the MAPK and NF-κB signals in OVA-induced asthmatic mice.; Authors - Yujiao Luo
human	PLoS One; Mesenchymal stromal cell-derived extracellular vesicles reduce lung inflammation and damage in nonclinical acute lung injury: Implications for COVID-19.; Authors - Caryn Cloer