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Animal-free Recombinant Human IFN gamma
Humankine®3 Publications

Catalog number: HZ-1301

HEK293 expressed Endotoxin-free Animal-component free

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Freight/Packing £00.00

Format: Lyophilized

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Technical Specifications

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IFN gamma activity assay

The activity was determined by dose dependent inhibition of proliferation of HT-29 cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

IFN gamma purity

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
3458
Species:
Human
Expression:
HEK293
Activity:
Typically ≤ 0.05 ng/mL EC50
Purity:
>95%
Molecular Mass:
29-32 kDa
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human albumin (HSA).

Product Description

Product Description:

Recombinant Human Interferon gamma (IFN γ), is a cytokine also known as type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ occurs as a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types such as the natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. Following secretion, the IFN γ dimer binds to its receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). The activation of the IFN γ receptor leads to activation of the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins.



Background:

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)



Synonyms:
IFNgamma



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