• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-23 protein

Activity

Typically ≤ 4 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1254

Synonyms

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF



Technical Specifications

GeneID 51561,3593
Species Human
Expression HEK293
Activity Typically ≤ 4 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberQ9NPF7
Molecular Mass 55 kDa, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin 23 (IL-23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of IL-23 identified initially are stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages (PMID: 16393998;15657292;12023369;12610626)

Synonyms

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF

Publications

SpeciesTitle
human

Ann Rheum Dis

JAK selectivity and the implications for clinical inhibition of pharmacodynamic cytokine signalling by filgotinib, upadacitinib, tofacitinib and baricitinib.

Authors - Paqui G Traves
human

Cell Rep

Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1.

Authors - YusukeEndo
human

Br J Pharmacol

The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammation

Authors - Yanhong Pan
human

J Mol Cell Cardiol

Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.

Authors - MakikoOhshima
human

J Immunol

The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species.

Authors - Raymond YYu
human

Sci Rep

Functional defects in CD4+ CD25high FoxP3+ regulatory cells in ankylosing spondylitis.

Authors - HuifangGuo