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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-7 protein

Activity

0.2-1.4 ng/mL EC50

Specific Activity

2.53x10^8 IU/mg calibrated against Human IL-7 reference standard (NIBSC cat no: 90/530). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1281

Synonyms

IL 7, IL7, IL-7, interleukin 7

Validation Data Gallery

  • The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • Recombinant human IL-7 (HZ-1281) stimulates dose-dependent proliferation of murine 2E8 human cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-1.4 ng/mL.

    Recombinant human IL-7 (HZ-1281) stimulates dose-dependent proliferation of murine 2E8 human cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-1.4 ng/mL.

Technical Specifications

GeneID 3574
Species Human
Expression HEK293
Activity 0.2-1.4 ng/mL EC50
Specific Activity2.53x10^8 IU/mg calibrated against Human IL-7 reference standard (NIBSC cat no: 90/530). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP10168
Molecular Mass 19, 25, and 30 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-7 (IL7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration (PMID: 29663382; 29655570; 29449560)

Synonyms

IL 7, IL7, IL-7, interleukin 7