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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-7 protein

Activity

Typically ≤ 1 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1281

Synonyms

IL 7, IL7, IL-7, interleukin 7

Validation Data Gallery

  • The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The specific activity was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The specific activity was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 3574
Species human
Expression HEK293
Activity Typically ≤ 1 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Molecular Mass 19, 25, and 30 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-7 (IL7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration (PMID: 29663382; 29655570; 29449560)

Synonyms

IL 7, IL7, IL-7, interleukin 7