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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human LIF protein

Activity

Typically ≤ 1 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1292

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Validation Data Gallery

  • The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The activity was determined by the dose­-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose­-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 3976
Species Human
Expression HEK293
Activity Typically ≤ 1 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP15018
Molecular Mass 35-53 kDa, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

LIF is a pleiotropic cytokine with a variety of roles. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface (PMID: 29620145; 29391217; 29271384)

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Publications

SpeciesTitle

Reprod Biol Endocrinol

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium.

Authors - Yue Liu
View Article
mouse

Nucleic Acids Res

Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs.

Authors - Mahmoud-Reza Rafiee
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Irina (Verified Customer) (07-01-2021)

LIF cytokine was successfully used at 10ng/ml concentration for the culture of mES cells and their maintenance in a undifferentiated state

  • Applications: Cell culture
  • Cell Tissue Type: mESCs