- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human LIF protein
Cat no : HZ-1292
Synonyms
CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI
Validation Data Gallery
Technical Specifications
GeneID | 3976 |
Species | Human |
Expression | HEK293 |
Activity | Typically ≤ 1 ng/mL EC50 |
Purity | >95% |
Endotoxin | <1 EU/μg |
Accession Number | P15018 |
Molecular Mass | 35-53 kDa, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
LIF is a pleiotropic cytokine with a variety of roles. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface (PMID: 29620145; 29391217; 29271384)
Synonyms
CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI
Publications
Species | Title |
---|---|
Reprod Biol Endocrinol Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium. | |
Nucleic Acids Res Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Irina (Verified Customer) (07-01-2021) | LIF cytokine was successfully used at 10ng/ml concentration for the culture of mES cells and their maintenance in a undifferentiated state
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