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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Sonic Hedgehog (SHH) protein


Typically ≤350 ng/mL EC50

Species Reactivity




Cat no : HZ-1306


HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

Technical Specifications

GeneID 6469
Species human
Expression HEK293
Activity Typically ≤350 ng/mL EC50
Purity >90%
Endotoxin<0.5 EU/μg
Molecular Mass 20-24 kDa
Formulation 10mM Tris pH 7.4 + 100mM NaCl + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).


SHH, also named as HHG-1, belongs to the hedgehog family. SHH binds to the patched receptor (PTC), that in association with smoothened (SMO) activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. SHH is synthesized as a 45 kDa protein precursor that is autocatalytically cleaved to yield of 20 kDa N-terminal fragment (24-197 in the human gene sequence) knowing as being responsible for all known hedgehog biological activity and a 25 kDa C-terminal fragment that contains the auto-processing machinery (PMID:10753901).


HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS