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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human Sonic Hedgehog (SHH)

Activity

Typically ≤350 ng/mL EC50

Species Reactivity

human

Purity

>90%

Cat no : HZ-1306

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS



Technical Specifications

GeneID 6469
Species human
Expression HEK293
Activity Typically ≤350 ng/mL EC50
Purity >90%
Molecular Mass 20-24 kDa
Formulation 10mM Tris pH 7.4 + 100mM NaCl + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Animal-free Recombinant Human SHH (Sonic Hedgehog) protein is the orthologue of drosophila hedgehog (Hh) and plays a critical role in vertebrate limb development. SHH gene encodes for a 45 kDa protein that undergoes autoproteolysis activated by its own C-terminal domain. Furthermore, the C-terminal domain also carries out cholesterol transferase activity. This auto-cleavage results in the formation of a 174 amino acid N-terminal product with covalently linked cholesterol moiety and a palmitoyl modification. The presence of the cholesterol and palmitoyl moieties is required for the activity of the mature SHH protein. These modifications are found in SHH derived from human cells, but are absent in bacterially expressed SHH. The N-terminal (active) form of SHH shares 98% aa homology to mouse, rat, canine, porcine and chicken SHH. It has been associated with tissue regeneration following injury and development of certain cancers in adults.

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS