ubiquitin Polyclonal antibody

ubiquitin Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)





Cat no : 10201-2-AP


Polyubiquitin B, UBB, ubiquitin, ubiquitin B

Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10201-2-AP targets ubiquitin in WB, IF, FC, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Arabidopsis, Trypanosoma cruzi, monkey, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ubiquitin fusion protein Ag0260
Full Name ubiquitin B
GenBank Accession NumberBC000379
Gene Symbol UBB
Gene ID (NCBI) 7314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.


Product Specific Protocols
WB protocol for ubiquitin antibody 10201-2-APDownload protocol
IHC protocol for ubiquitin antibody 10201-2-APDownload protocol
IF protocol for ubiquitin antibody 10201-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


David (Verified Customer) (01-02-2024)

Various bands in the soluble fraction, potentially from conjugated proteins, but also clear bands for the ubiquitin monomers.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y lysate

Brice-Emmanuel (Verified Customer) (10-12-2023)

Work well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: Mouse Heart Tissue
ubiquitin Antibody Western Blot validation (1/500 dilution) in Mouse Heart Tissue (Cat no:10201-2-AP)

Brandon (Verified Customer) (03-14-2022)

The antibody works perfectly for ubiquitin located in Human Fibroblast

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HFFs
ubiquitin Antibody Western Blot validation (1:1000 dilution) in HFFs (Cat no:10201-2-AP)

Anita (Verified Customer) (08-06-2021)

10ug protein load from C. elegans

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C. elegans
ubiquitin Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:10201-2-AP)

Azita (Verified Customer) (06-02-2021)

Western blot analysis using ubiquitin polyclonal antibody in NSC34 cell line at dilution of 1:500.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: NSC34

Robert (Verified Customer) (06-24-2019)

1. reducing conditions2. non-reducing conditions

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
ubiquitin Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:10201-2-AP)