|Positive WB detected in||MDA-MB-453s cells, MCF-7 cells, mouse liver tissue|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10201-2-AP targets ubiquitin in WB, IHC, IF, FC, CoIP, chIP applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Arabidopsis, human, monkey, mouse, rat, Trypanosoma cruzi|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ubiquitin fusion protein Ag0260|
|Full Name||ubiquitin B|
|Calculated molecular weight||26 kDa|
|GenBank accession number||BC000379|
|Gene ID (NCBI)||7314|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anita (Verified Customer) (08-06-2021)
10ug protein load from C. elegans
Azita (Verified Customer) (06-02-2021)
Western blot analysis using ubiquitin polyclonal antibody in NSC34 cell line at dilution of 1:500.
Robert (Verified Customer) (06-24-2019)
1. reducing conditions2. non-reducing conditions