ubiquitin Polyclonal antibody

ubiquitin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10201-2-AP

Synonyms

Polyubiquitin B, UBB, ubiquitin, ubiquitin B



Tested Applications

Positive WB detected inMDA-MB-453s cells, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman pancreas tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

10201-2-AP targets ubiquitin in WB, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Arabidopsis thaliana, human, monkey, mouse, rat, T. cruzi
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ubiquitin fusion protein Ag0260
Full Name ubiquitin B
Calculated molecular weight 26 kDa
Observed molecular weight 8 kDa, 24 kDa
GenBank accession numberBC000379
Gene symbol UBB
Gene ID (NCBI) 7314
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

Protocols

Product Specific Protocols
WB protocol for ubiquitin antibody 10201-2-APDownload protocol
IHC protocol for ubiquitin antibody 10201-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Authors - Xi Shen
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun

Sci Adv

The endogenous retrovirus-derived long noncoding RNA TROJAN promotes triple-negative breast cancer progression via ZMYND8 degradation.

Authors - Xi Jin
humanWB

Nat Commun

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Authors - Ling Guo
humanWB,chIP

Mol Cancer

Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.

Authors - Qiang Cai
mouseWB

Sci Adv

The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation.

Authors - Xin Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Robert (Verified Customer) (06-24-2019)

1. reducing conditions 2. non-reducing conditions

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
ubiquitin Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:10201-2-AP)