Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10201-2-AP targets ubiquitin in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, canine, monkey, chicken, bovine, c.elegans, arabidopsis |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0260 Product name: Recombinant human ubiquitin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 104-229 aa of BC000379 Sequence: AKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGC Predict reactive species |
| Full Name | ubiquitin B |
| GenBank Accession Number | BC000379 |
| Gene Symbol | ubiquitin |
| Gene ID (NCBI) | 7314 |
| RRID | AB_671515 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0CG47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ubiquitin antibody 10201-2-AP | Download protocol |
| IF protocol for ubiquitin antibody 10201-2-AP | Download protocol |
| IHC protocol for ubiquitin antibody 10201-2-AP | Download protocol |
| WB protocol for ubiquitin antibody 10201-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
10201-2-AP is an anti-Ubiquitin antibody with 1,107 total citations. Citations appear in high-impact journals including Nature, Cell, Cancer Cell, Signal Transduction and Targeted Therapy, Nature Communications, Cell Death & Differentiation, Autophagy, Advanced Science, and Theranostics. Research fields span cancer biology, protein ubiquitination and degradation, autophagy, ferroptosis, metabolism, neurodegeneration, immunology, cardiology, bone biology, liver disease, drug resistance, and plant biology, reflecting the antibody's broad utility in studying ubiquitin-mediated processes across diverse biological systems.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Glucosidase alpha neutral C promotes influenza virus replication by inhibiting proteosome-dependent degradation of hemagglutinin | ||
Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease | ||
Signal Transduct Target Ther Lysophosphatidylethanolamine 18:1 drives clear cell renal cell carcinoma by stabilizing SIRT6 to reprogram lipid metabolism. | ||
Nature Proteasome inhibition for treatment of leishmaniasis, Chagas disease and sleeping sickness. | ||
Cancer Cell Targeting tumor-intrinsic STK40 induces immune vulnerability and drives T cell reinvigoration. |
Reviews
What customers say
Across 8 reviews, this ubiquitin antibody received an average rating of approximately 4.4/5. Most users reported successful performance in Western blot, with dilutions ranging from 1:500 to 1:1000 across diverse samples including human fibroblasts, Caco-2 cells, SH-SY5Y lysates, mouse heart tissue, C. elegans, NSC34 cells, and porcine ST cells. Several reviewers highlighted reliable results on the first attempt. One user noted additional bands in the soluble fraction, possibly from conjugated proteins, though clear ubiquitin monomer bands were still visible. The antibody was also used for immunofluorescence and immunohistochemistry with positive outcomes.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Viktoria (Verified Customer) (06-05-2026) | Worked perfect, already on the first try!
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P (Verified Customer) (07-15-2025) | Used for caco2 cells and animal colon tissue
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David (Verified Customer) (01-02-2024) | Various bands in the soluble fraction, potentially from conjugated proteins, but also clear bands for the ubiquitin monomers.
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Brice-Emmanuel (Verified Customer) (10-12-2023) | Work well
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Brandon (Verified Customer) (03-14-2022) | The antibody works perfectly for ubiquitin located in Human Fibroblast
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Anita (Verified Customer) (08-06-2021) | 10ug protein load from C. elegans
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Azita (Verified Customer) (06-02-2021) | Western blot analysis using ubiquitin polyclonal antibody in NSC34 cell line at dilution of 1:500.
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Robert (Verified Customer) (06-24-2019) | 1. reducing conditions2. non-reducing conditions
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