ubiquitin Polyclonal antibody
ubiquitin Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF, FC, CoIP, ChIP, ELISA
Conjugate
Unconjugated
327
Cat no : 10201-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MDA-MB-453s cells, MCF-7 cells, mouse liver tissue |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 262 publications below |
IHC | See 9 publications below |
IF | See 16 publications below |
CoIP | See 20 publications below |
ChIP | See 1 publications below |
Product Information
10201-2-AP targets ubiquitin in WB, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, Arabidopsis, Trypanosoma cruzi, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ubiquitin fusion protein Ag0260 |
Full Name | ubiquitin B |
GenBank accession number | BC000379 |
Gene symbol | UBB |
Gene ID (NCBI) | 7314 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.
Protocols
Product Specific Protocols | |
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WB protocol for ubiquitin antibody 10201-2-AP | Download protocol |
IHC protocol for ubiquitin antibody 10201-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Proteasome inhibition for treatment of leishmaniasis, Chagas disease and sleeping sickness. | ||
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
Nat Commun UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean | ||
Acta Neuropathol Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases. | ||
Mol Cancer Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Brice-Emmanuel (Verified Customer) (10-12-2023) | Work well
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FH Brandon (Verified Customer) (03-14-2022) | The antibody works perfectly for ubiquitin located in Human Fibroblast
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FH Anita (Verified Customer) (08-06-2021) | 10ug protein load from C. elegans
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FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using ubiquitin polyclonal antibody in NSC34 cell line at dilution of 1:500.
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FH Robert (Verified Customer) (06-24-2019) | 1. reducing conditions2. non-reducing conditions
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