47 results for: "immunoprecipitation" Filters Application Note How to conduct a Co-immunoprecipitation (Co-IP) Co-IP describes the immunoprecipitation of a protein of interest and its interacting partners. Application Note Immunoprecipitation without additional bands Pull-down of proteins with Nano-Traps Featured Products GFP-Trap for immunoprecipitation Application Note Immunoprecipitation of Myc-tagged proteins – How it works ChromoTek Myc-Trap® is an optimized tool for immunoprecipitation (IP/Co-IP) Protocol Introduction to Immunoprecipitation An overview of the immunoprecipitation (IP) application. Protocol Immunoprecipitation Workflow Step by step immunoprecipitation workflow with buffer optimization tips. Protocol Immunoprecipitation ppt A slideshow overview of our IP technical guide. Application Note Tips and tricks for immunoprecipitation of low abundant proteins For a successful IP, the abundance or expression level of the POI is crucial. If a POI is strongly expressed and highly abundant, then the IP will most likely be straightforward, with no difficulties. Application Note Antibody Specificity and affinity in Immunoprecipitation What is antibody specificity and affinity? And why is it important for immunoprecipitation? Application Note Which beads should I use for my immunoprecipitation? In detail, all beads have very low background binding due to the highly specific binding of the Nano-Traps. Application Note The best anti-GFP antibody for immunoprecipitation: GFP-Trap Comparison of GFP-Trap and anti-GFP IgG antibody Application Note 5 Tips for better immunoprecipitation (IP) Application Note Co-immunoprecipitation troubleshooting, tips and tricks Co-immunoprecipitation (Co-IP) describes the isolation of a protein and its binding partners from a cell extract using a Nanobody or antibody that is bound to beads. The protein, that directly interacts with the Nanobody, or antibody beads is called “bait”. The binding partner that is indirectly precipitated is called “prey”. Application Note 8 Top Tips For Immunoprecipitation 8 tips on how to perform better IP experiments. Technical Talk Save hours on your immunoprecipitation experiments Save hours on your immunoprecipitation (IP) experiments Application Note Advantages and limitations of different antibody formats in immunoprecipitation Application Note What GFP-Trap should I use for my immunoprecipitation? GFP-Trap Agarose, GFP-Trap Magnetic Agarose,GFP-Trap Magnetic Particles M-270, GFP-Trap 96 Multiwell Plate Application Note On-bead digestion protocol: From immunoprecipitation with nanobodies to MS analysis Follow the on-bead digestion protocol for effective mass spectrometry analysis of (co-) precipitated proteins Protocol IP Preparation Immunoprecipitation tips including binding proteins and beads and tips on choosing the right primary antibody for IP. Technical Talk All about Immunoprecipitation Workshop Protocol IP Troubleshooting Troubleshooting tips and solutions for common IP problems. Protocol ChIP Protocol A detailed 4 day step by step protocol for Chromatin Immunoprecipitation (ChIP) to help you achieve optimal results. Application Note GFP-Trap for immunoprecipitation (IP) – stringent washing The high stability of the GFP-Trap® enables its use in virtually any lysis and wash buffer. Application Note Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins Publications using ChromoTek's GST-Trap in various applications Application Note How to obtain a low background in immunoprecipitation assays Tips and tricks for better IP results with GFP-Trap Technical Talk Why GFP-Trap gives the best immunoprecipitation results Why GFP-Trap gives the best immunoprecipitation (IP) results Featured Products New anti-mNeonGreen antibody & Nano-Trap for Immunofluorescence & Immunoprecipitation Novel tools for the analysis of mNeonGreen fusion proteins Application Note Myc-Trap for both immunoprecipitation and affinity purification of Myc-tagged proteins The Alpaca antibody advantage for pull-down of Myc-tagged proteins Protocol Considerations before starting your IP experiment Learn the importance of antibody titration in order to determine the optimal signal-to-noise ratio in IP. Application Note How to elute GFP-fusion protein from the GFP-Trap® Tips and tricks Protocol IP Sample Preparation Learn about the best sample preparation and lysis buffer for your IP experiments. Application Note Spot Capture and Detection Peptide Tag and Nanobody The Spot Nanobody for multiple capture & detection applications of Spot-tagged proteins Application Note Spot-Trap or Spot-Cap? Our Sales Rep answers one of the most frequent customer questions Application Note Why is the Fab-Trap™ so Fab-ulous? ChromoTek’s Fab-Trap™ is an IP reagent based on an antibody Fab-fragment. Application Note How to immunoprecipitate Flag®-tagged proteins Flag®-tag (or DYKDDDDK-tag) is a commonly used short peptide tag for multiple applications such as immunoprecipitation (IP), protein purification, immunofluorescence, and Western blotting (WB). Application Note Flag-tag and 3x Flag-tag Overview about specifications, properties, origin, sequence, size, specifications, etc. of Flag-tag Research Area Virus research using GFP References from the last 4 years in virus research using GFP and ChromoTek's GFP-Trap Research Area Overview of the most recent Spot Peptide-Tag & Spot-Nanobody publications ChromoTek’s Spot-Tag system for universal capture & detection applications such as immunofluorescence, immunoprecipitation, and protein purification Application Note Multiple kits for streamlined affinity purification mass spectrometry (AP-MS) iST Nano-Trap kits simplify immunoprecipitation/Co-immunoprecipitation and preparation of samples for mass spectrometry-based proteomics. Research Area Mapping the mitotic protein interactome of Drosophila embryos with nanobodies Immunoprecipitation of Protein Complexes from Drosophila Embryos with GFP-Trap Application Note V5-Tag: Properties V5-Tag: Properties, origin, specifications, antibodies & Nanobodies Application Note Seizing the day: How to save time on your immunoassay experiments When time in the lab is limited, learn how to save precious hours on your IP, IF, and western blotting experiments. Application Note Chromatin Immunoprecipitation (ChIP) with RFP-tagged or mCherry-tagged proteins RFP-Trap for ChIP analysis in order to detect enrichment of mCherry-tagged proteins at DNA binding sites Application Note How to prepare protein interaction partners for MS analysis Combined use of ChromoTek's GFP-Trap for immunoprecipitation and PreOmics iST sample preparation kit for reproducible identification of proteins and interaction partners. Application Note Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification. Myc-tag is a peptide tag derived from the c-Myc protein. The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, immunofluorescence and protein purification. Research Area How fluorescent proteins can be applied in SARS-CoV-2 research GFP or RFP and their derivatives are commonly used for virus research Application Note Fluorescent protein tags Types of fluorescent protein tags and comparisons