Various lysates were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

1X10^6 HUVEC cells were stained with 0.2ug VEGF Receptor 2 antibody (26415-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

IP result of anti-VEGFR2/KDR (IP:26415-1-AP, 4ug; Detection:26415-1-AP 1:3000) with human placenta tissue lysate 600 ug.

human placenta tissue were subjected to SDS PAGE followed by western blot with 67407-1-Ig (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

1X10^6 HUVEC cells were intracellularly stained with 0.20 ug/test Anti-Human VEGFR2 (67407-1-Ig, Clone:1G2A8) (red) or 0.20 ug Mouse IgG1 Isotype Control(66360-1-Ig, CloneT1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

1x10^6 HUVEC cells were surface stained with 0.25 ug Anti-Human VEGFR2/CD309 (98009-1-RR, Clone: 230238B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

Surface Plasmon Resonance (SPR) kinetic assays of 98009-1-RR against Human VEGFR2/CD309 were performed. The affinity constant is 46.58 nM.

1x10^6 HUVEC cells were surface stained with 0.25 ug Anti-Human VEGFR2/CD309 (98009-1-PBS, Clone: 230238B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

1x10^6 HUVEC cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VEGFR2/KDR (CL488-67407, Clone:1G2A8)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were not fixed.

Standard curve of MP50080-1

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (KDR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.