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  • KD/KO Validated

VEGFR2 Polyclonal antibody

VEGFR2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26415-1-AP

Synonyms

CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGF Receptor 2, VEGFR, VEGFR 2, VEGFR2



Tested Applications

Positive WB detected inhuman placenta tissue, mouse heart tissue
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26415-1-AP is VEGFR2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFR2 fusion protein Ag24589
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Calculated molecular weight 1356 aa, 152 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC131822
Gene symbol KDR
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Protocols

Product Specific Protocols
WB protocol for VEGFR2 antibody 26415-1-APDownload protocol
FC protocol for VEGFR2 antibody 26415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int J Biochem Cell Biol

Exosomes from hypoxia-treated human adipose-derived mesenchymal stem cells enhance angiogenesis through VEGF/VEGF-R.

Authors - Yudi Han
humanWB,IHC

BMC Cancer

VEGFR2 promotes tumorigenesis and metastasis in a pro-angiogenic-independent way in gastric cancer.

Authors - Lian Lian
  • KD Validated
ratWB

CNS Neurosci Ther

Dl-3-N-butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats.

Authors - Pan-Ting Zhou
sheepWB,IHC

Theriogenology

Effects of l-arginine on endometrial microvessel density in nutrient-restricted Hu sheep.

Authors - Xiao-Xiao Gao
humanIF

J Clin Med

Human Colorectal Cancer Infrastructure Constructed by the Glycocalyx.

Authors - Masahito Tachi
ratIF

Ann Transl Med

MiR-92a regulates endothelial progenitor cells (EPCs) by targeting GDF11 via activate SMAD2/3/FAK/Akt/eNOS pathway.

Authors - Hai-Tao Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on angiogenesis study and use this for WB on HUVECs. It works very well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs