VEGFR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

VEGFR2 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

62

Reviews (1)

Cat no : 26415-1-AP

Synonyms

CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGF Receptor 2, VEGFR, VEGFR 2, VEGFR2

---

Validation Data Gallery

• 

  WB analysis using 26415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 26415-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26415-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HUVEC using 26415-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug VEGF Receptor 2 antibody (26415-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Tested Applications

• Positive WB detected in: human placenta tissue, mouse heart tissue, mouse lung tissue, mouse placenta tissue, rat lung tissue
• Positive FC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 43 publications below
• IHC: See 10 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

The immunogen of 26415-1-AP is VEGFR2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFR2 fusion protein Ag24589
• Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Calculated molecular weight: 1356 aa, 152 kDa
• Observed molecular weight: 200 kDa
• GenBank accession number: BC131822
• Gene symbol: KDR
• Gene ID (NCBI): 3791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Publications

Species	Application	Title
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
human	WB	Bioact Mater; Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.; Authors - Wenjing Liu
mouse	IHC	Cancer Lett; PI3K inhibitor impairs tumor progression and enhances sensitivity to anlotinib in anlotinib-resistant osteosarcoma.; Authors - Chenglong Chen
human	WB	Cancer Lett; Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner.; Authors - Jiao Li ; KD Validated
rat	IF	Bioengineered; The crescendo pulse frequency of shear stress stimulates the endothelialization of bone marrow mesenchymal stem cells on the luminal surface of decellularized scaffold in the bioreactor; Authors - Yuhao Jiao
human	WB,IF	PLoS Pathog; Kaposi's sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis; Authors - Weikang Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on angiogenesis study and use this for WB on HUVECs. It works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs

Related Products

VEGFA Polyclonal antibody

19003-1-AP

VEGFR-1/FLT-1 Polyclonal antibody

13687-1-AP

Neuropilin 1 Monoclonal antibody

60067-1-Ig

VEGFR-1/FLT-1 Polyclonal antibody

13687-1-AP

EGFR-Specific Polyclonal antibody

18986-1-AP

EGFR (N-terminal) Polyclonal antibody

22542-1-AP

SRC Polyclonal antibody

11097-1-AP

SHP2 Polyclonal antibody

20145-1-AP