Anti-Human VEGFR2/CD309 Rabbit Recombinant Antibody

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  FC experiment of HUVEC using 98009-1-RR

  1x10^6 HUVEC cells (left) or HeLa cells (right) were surface stained with 0.25 ug Anti-Human VEGFR2/CD309 Rabbit RecAb (98009-1-RR, Clone: 230238B11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98009-1-RR against Human VEGFR2/CD309 were performed. The affinity constant is 30.6 nM.

VEGFR2/CD309 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98009-1-RR

Clone No.230238B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

KDR, VEGFR2, VEGFR2/KDR, 230238B11, CD309

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Tested Applications

• Positive FC detected in: HUVEC cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98009-1-RR targets VEGFR2/CD309 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VEGFR2/CD309 fusion protein Eg0218
• Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Calculated Molecular Weight: 152 kDa
• GenBank Accession Number: NM_002253
• Gene Symbol: KDR
• Gene ID (NCBI): 3791
• RRID: AB_3672151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35968-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

VEGFR2, also named as CD309, KDR and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.