Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66005-1-Ig (6*His, His-Tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (66005-1-Ig) at various dilutions.

IP result of anti-6*His, His-Tag (IP:66005-1-Ig, 7ug; Detection:66005-1-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 6*His, His-Tag antibody (66005-1-Ig, Clone: 1B7G5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66005 (6*His, His-Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (10001-0-AP) at various dilutions.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647-conjugated 6*His, His-Tag antibody (CL647-66005, Clone: 1B7G5 ) at dilution of 1:500.

1X10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-N/A 6*His, His-Tag (CL647-66005, Clone:1B7G5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-N/A 6*His, His-Tag (CL647-66005, Clone:1B7G5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66005-1-Ig (6*His, His-Tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (66005-1-Ig) at various dilutions. This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

IP result of anti-6*His, His-Tag (IP:66005-1-Ig, 7ug; Detection:66005-1-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 6*His, His-Tag antibody (66005-1-Ig, Clone: 1B7G5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

Biotin-66005 was tested by ELISA. Ag19172(GST-3*MYC-6*HIS-3*FLAG-6*HIS-3*HA-6*HIS)was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of Biotin-66005 (start dilution 1:1000. Bound antibodies were detected with Streptavidin Poly-HRP(1:5000)followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm.

Various lysates were subjected to SDS PAGE followed by western blot with CL750-66005 (6*His, His-Tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66005 (6*His, His-Tag antibody), at dilution of 1:100.