6*his,His tag Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 10001-0-AP

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Freight/Packing

Con: 60 μg/150 μl

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Tested Applications

Applications:
ELISA, WB
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Species specificity:
recombinant protein

Positive WB detected in:
Recombinant protein

Recommended dilution:
WB : 1:500-1:2000

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Recombinant Protein

Full name:
6*his,His tag

Calculated molecular weight:
1 kDa

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
6 his, 6*his,His tag, his tag
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.


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