6*his,His tag Polyclonal antibody

6*his,His tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (5)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10001-0-AP

Synonyms

6 his, 6*his,His tag, his tag


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  • 6*his,His tag Antibody WB recombinant protein  10001-0-AP

    WB analysis of recombinant protein using 10001-0-AP

    Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (10001-0-AP) at various dilutions.

"6*his,His tag Antibodies" Comparison

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Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10001-0-AP targets 6*his,His tag in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name 6*his,His tag
Calculated Molecular Weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_11232228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.

Publications

SpeciesApplicationTitle
ChIP

Nature

High-resolution landscape of an antibiotic binding site

Authors - Kevin B Yang
WB

Am J Respir Crit Care Med

Targeting Procalcitonin Protects Vascular Barrier Integrity

Authors - Laura Brabenec
WB

Nat Commun

USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.

Authors - Shangda Yang
WB

Nat Commun

Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery.

Authors - Jun Zhou

Nat Commun

Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome.

Authors - Lianhui Sun

Cell Rep

p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP

Authors - Yingdan Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Brogan (Verified Customer) (11-09-2022)

Works very well, will buy again

  • Applications: Western Blot, Other
FH

Jean-Baptiste (Verified Customer) (04-21-2022)

Tested in Western blot for analysing the production in bacteria and the purification of a recombinant his-tagged protein. Worked quite well, with few unspecific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
6*his,His tag Antibody Western Blot validation (1:2000 dilution) in (Cat no:10001-0-AP)