6*his,His tag Antibody

6*his,His tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10001-0-AP

Synonyms

6 his, 6*his,His tag, his tag



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

10001-0-AP targets 6*his,His tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

Reactivity recombinant protein
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name 6*his,His tag
Calculated molecular weight 1 kDa
Observed molecular weight
GenBank accession number
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.

Publications

SpeciesApplicationTitle
WB

Protein J

Crystal structure of Human ASB9-2 and substrate-recognition of CKB.

Authors - Fei Xiangwei X

Arch Insect Biochem Physiol

The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori.

Authors - Yafei Ma
WB

PeerJ

Codon optimization, expression in Escherichia coli, and immunogenicity analysis of deformed wing virus (DWV) structural protein.

Authors - Dongliang Fei
humanWB

Onco Targets Ther

Fibroblast Activation Protein (FAP) Overexpression Induces Epithelial-Mesenchymal Transition (EMT) in Oral Squamous Cell Carcinoma by Down-Regulating Dipeptidyl Peptidase 9 (DPP9).

Authors - Qing-Qing Wu
WB

Virology

An important amino acid in nucleoprotein contributes to influenza A virus replication by interacting with polymerase PB2.

Authors - Xun Gui
WB

Exp Cell Res

Silencing of OSBP-related protein 8 (ORP8) modifies the macrophage transcriptome, nucleoporin p62 distribution, and migration capacity.

Authors - Béaslas Olivier O