6*His, His-Tag Monoclonal antibody, PBS Only

6*His, His-Tag Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B7G5

Cat no : 66005-1-PBS

Synonyms

6*His, 6*His, His Tag, 6*His, His-Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED

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Validation Data Gallery

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  WB analysis of recombinant protein using 66005-1-Ig (same clone as 66005-1-PBS)

  Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (66005-1-Ig) at various dilutions. This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

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  IP experiment of Transfected HEK-293 using 66005-1-Ig (same clone as 66005-1-PBS)

  IP result of anti-6*His, His-Tag (IP:66005-1-Ig, 7ug; Detection:66005-1-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 66005-1-Ig (same clone as 66005-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66005-1-Ig (6*His, His-Tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 66005-1-Ig (same clone as 66005-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 6*His, His-Tag antibody (66005-1-Ig, Clone: 1B7G5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66005-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66005-1-PBS targets 6*His, His-Tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: 6*His, His-Tag
• Calculated Molecular Weight: 1 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.