1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg CoraLite® Plus 555 conjugated-LAG3 VHH (CL555-lt). Cells were not fixed.

Live HeLa cells transfected with human LAG3 were immunostained with CoraLite® Plus 555 conjugated-LAG3 VHH (CL555-lt, 1:500, yellow). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with CL555-lt can also be performed after formaldehyde fixation of the cells.

1X10^6 CHO TIGIT stable expressing cells (red) and CHO parental cells were surface stained with 0.25 µg CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt). Cells were not fixed.

1x10^6 PBMC were stained with 1ug CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt) and 5ul CoraLite® Plus 405 conjugated Anti-Human CD3 (Clone:UCHT1; CL405-65151). Lymphocytes were gated.

Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with CL555-tgt can also be performed after formaldehyde fixation of the cells.

Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, orange). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.  Note: Immunostaining with CL555-tgt can also be performed after formaldehyde fixation of the cells.

Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.

Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

1X10^6 CHO TIM3 stable expressing cells (red) and CHO parental cells were surface stained with 0.5 µg CoraLite® Plus 555 conjugated-TIM3 VHH (CL555-tmt). Cells were not fixed.

1X10^6 CHO TIM3 stable expressing cells (red) were surface stained with 0.5 µg CoraLite® Plus 555 conjugated-TIM3 VHH (CL555-tmt). Cells were not fixed and pre-gated on co-expressed, GFP positive cells.

Live HeLa cells transfected with human TIM-3 were immunostained with CoraLite® Plus 555 conjugated-TIM3 VHH (CL555-tmt, 1:1000, yellow). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with CL555-tmt can also be performed after formaldehyde fixation of the cells.

Live HeLa cells transfected with human TIM-3 were immunostained with CoraLite® Plus 555 conjugated-TIM3 VHH (CL555-tmt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, intracellular pool of TIM-3 was immunostained with CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt, 1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt). Cells were not fixed.

Live HeLa cells transfected with human LAG3 were immunostained with CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt, 1:500, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with CL647-lt can also be performed after formaldehyde fixation of the cells.

1X10^6 CHO TIGIT stable expressing cells (red) and CHO parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt). Cells were not fixed.

1x10^6 PBMC were stained with 1ug CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt) and 5ul CoraLite® Plus 405 conjugated Anti-Human CD3 (Clone:UCHT1; CL405-65151). Lymphocytes were gated.

Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.  Note: Immunostaining with CL647-tgt can also be performed after formaldehyde fixation of the cells.

Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired and post-processed with Leica Thunder Imager, 40x objective.

Live HeLa cells transfected with human TIGIT were immunostained with CoraLite® Plus 555 conjugated-TIGIT VHH (CL555-tgt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, the intracellular pool of TIGIT was immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

Live HeLa cells transfected with human TIGIT were probed with human recombinant anti-TIGIT IgG. After formaldehyde fixation, the cells were immunostained with CoraLite® Plus 647 conjugated-TIGIT VHH (CL647-tgt,1:1000, magenta) and with anti-human IgG Nano-Secondary (shuGCL488-2, green). Cell nuclei were stained with DAPI (blue).  Epifluorescence images were acquired with 20x objective and post-processed.

1X10^6 CHO TIM3 stable expressing cells (red) and CHO parental cells were surface stained with 0.5 µg CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt). Cells were not fixed.

1X10^6 CHO TIM3 stable expressing cells (red) were surface stained with 0.5 µg CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt). Cells were not fixed and pre-gated on co-expressed, GFP positive cells.

Live HeLa cells transfected with human TIM-3 were immunostained with CoraLite® Plus 555 conjugated-TIM3 VHH (CL555-tmt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, intracellular pool of TIM-3 was immunostained with CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt, 1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.

Live HeLa cells transfected with human TIM-3 were immunostained with CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt, 1:1000, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with CL647-tmt can also be performed after formaldehyde fixation of the cells.

Live HeLa cells transfected with human TIM-3 were probed with cobolimab biosimilar (anti-TIM3, huIgG4). After formaldehyde fixation, the cells were immunostained with CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt, 1:1000, magenta) and with anti-human IgG Nano-Secondary (shuGCL488-2, green). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with 20x objective and post-processed.

1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg FITC Plus conjugated-LAG3 VHH (FITC-lt). Cells were not fixed.

Live HeLa cells transfected with human LAG3 were immunostained FITC Plus conjugated-LAG3 VHH (FITC-lt, 1:500, green). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with FITC-lt can also be performed after formaldehyde fixation of the cells.

1X10^6 HEK TIGIT transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg FITC Plus conjugated-TIGIT VHH (FITC-tgt). Cells were not fixed.

1x10^6 PBMC were stained with 1ug FITC Plus conjugated-TIGIT VHH (FITC-tgt) and 5ul CoraLite® Plus 405 conjugated Anti-Human CD3 (Clone:UCHT1; CL405-65151). Lymphocytes were gated.

Live HeLa cells transfected with human TIGIT were immunostained with FITC Plus conjugated-TIGIT VHH (FITC-tgt,1:1000, green). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with FITC-tgt can also be performed after formaldehyde fixation of the cells.