• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ChromoTek CoraLite® Plus 647-conjugated LAG3 VHH

Alpaca anti-LAG3 VHH for FC and IF. CL647-lt targets LAG3 in FC and IF applications and shows reactivity with Human samples.

Specificity/Target

LAG3-Tag

Applications

FC, IF

Type

Nanobody

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-lt

Synonyms

LAG3-Nanobody, LAG3-single domain antibody (sdAb), CD223, FDC, LAG 3, LAG3, LAG-3, lymphocyte activation gene 3, Protein FDC

Validation Data Gallery

  • 1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt). Cells were not fixed.

    1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt). Cells were not fixed.

  • Live HeLa cells transfected with human LAG3 were immunostained with CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt, 1:500, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with CL647-lt can also be performed after formaldehyde fixation of the cells.

    Live HeLa cells transfected with human LAG3 were immunostained with CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt, 1:500, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with CL647-lt can also be performed after formaldehyde fixation of the cells.

Product Information

Alpaca anti-LAG3 VHH for FC and IF. CL647-lt targets LAG3 in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateCoraLite® Plus 647 Fluorescent Dye
Type Nanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight15.141 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
RRID/
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA
Storage ConditionStore at -20°C; Shipped at ambient temperature.