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ChromoTek FITC Plus-conjugated LAG3 VHH

Alpaca anti-LAG3 VHH for FC and IF. FITC-lt targets LAG3 in FC and IF applications and shows reactivity with Human samples.

Specificity/Target

LAG3-Tag

Applications

FC, IF

Type

Nanobody

Conjugate

FITC Plus Fluorescent dye

Cat no : FITC-lt

Synonyms

LAG3-Nanobody, LAG3-single domain antibody (sdAb), CD223, FDC, LAG 3, LAG3, LAG-3, lymphocyte activation gene 3, Protein FDC

Validation Data Gallery

  • 1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg FITC Plus conjugated-LAG3 VHH (FITC-lt). Cells were not fixed.

    1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg FITC Plus conjugated-LAG3 VHH (FITC-lt). Cells were not fixed.

  • Live HeLa cells transfected with human LAG3 were immunostained FITC Plus conjugated-LAG3 VHH (FITC-lt, 1:500, green). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with FITC-lt can also be performed after formaldehyde fixation of the cells.

    Live HeLa cells transfected with human LAG3 were immunostained FITC Plus conjugated-LAG3 VHH (FITC-lt, 1:500, green). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with FITC-lt can also be performed after formaldehyde fixation of the cells.

Product Information

Alpaca anti-LAG3 VHH for FC and IF. FITC-lt targets LAG3 in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateFITC Plus Fluorescent dye
Type Nanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight15.141 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
RRID/
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA
Storage ConditionStore at -20°C; Shipped at ambient temperature.