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  • KD/KO Validated

14-3-3 Epsilon Polyclonal antibody

14-3-3 Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11648-2-AP

Synonyms

14 3 3 protein epsilon, 14 3 3E, 14-3-3, 14-3-3 epsilon, KCIP 1, MDCR, MDS, YWHAE



Tested Applications

Positive WB detected inA375 cells, HeLa cells
Positive IP detected inA375 cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11648-2-AP targets 14-3-3 Epsilon in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 14-3-3 Epsilon fusion protein Ag2247
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Calculated molecular weight 255 aa, 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC000179
Gene symbol YWHAE
Gene ID (NCBI) 7531
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

14-3-3 Epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 Epsilon.

Protocols

Product Specific Protocols
WB protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
IHC protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
IF protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
IP protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
FC protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Exp Clin Cancer Res

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Authors - Jia Song
humanWB,IF

Oncol Lett

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Authors - Kun Lu
humanWB

BMC Cancer

CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.

Authors - Hongbin Li
  • KD Validated

Sci Rep

Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis.

Authors - Frankie H F Lee
humanWB,IF

Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos
humanWB

Biochem Pharmacol

1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.

Authors - Yuhang Zhou