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14-3-3 epsilon Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 11648-2-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

14-3-3 epsilon Antibody WB A375 cells 11648-2-AP

A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP( 14-3-3 epsilon antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours

14-3-3 epsilon Antibody IP A375 cells 11648-2-AP

IP Result of anti-14-3-3 epsilon (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.

14-3-3 epsilon Antibody IF HepG2 cells 11648-2-AP

Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

14-3-3 epsilon Antibody IHC human lung cancer tissue 11648-2-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11648-2-AP( 14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

14-3-3 epsilon Antibody IHC human lung cancer tissue 11648-2-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11648-2-AP( 14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

14-3-3 epsilon Antibody FC HepG2 cells 11648-2-AP

1X10^6 HepG2 cells were stained with 0.2ug 14-3-3 epsilon antibody (11648-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
A375 cells, HeLa cells
Positive IP detected in:
A375 cells
Positive IHC detected in:
human lung cancer tissue, human gliomas tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
14-3-3 epsilon fusion protein Ag2247
Full name:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Calculated molecular weight:
255aa,29 kDa
Observed molecular weight:
29-32 kDa
GenBank accession number:
BC000179
Gene ID (NCBI):
7531
Gene symbol
YWHAE
Synonyms
14 3 3 protein epsilon, 14 3 3E, 14-3-3, 14-3-3 epsilon, KCIP 1, MDCR, MDS, YWHAE

Background

14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 epsilon.


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