- Featured Product
- KD/KO Validated
14-3-3 Epsilon Polyclonal antibody
14-3-3 Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 11648-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, HeLa cells |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human lung cancer tissue, human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
11648-2-AP targets 14-3-3 Epsilon in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 14-3-3 Epsilon fusion protein Ag2247 |
| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide |
| Calculated molecular weight | 255 aa, 29 kDa |
| Observed molecular weight | 29-32 kDa |
| GenBank accession number | BC000179 |
| Gene symbol | YWHAE |
| Gene ID (NCBI) | 7531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
14-3-3 Epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 Epsilon.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for 14-3-3 Epsilon antibody 11648-2-AP | Download protocol |
| IHC protocol for 14-3-3 Epsilon antibody 11648-2-AP | Download protocol |
| IF protocol for 14-3-3 Epsilon antibody 11648-2-AP | Download protocol |
| IP protocol for 14-3-3 Epsilon antibody 11648-2-AP | Download protocol |
| FC protocol for 14-3-3 Epsilon antibody 11648-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling. | ||
Oncol Lett 14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells. | ||
BMC Cancer CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.
| ||
Sci Rep Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis. | ||
Biochem Pharmacol 1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway. |
