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P glycoprotein Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 22336-1-AP

1 customer review

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Con: 72 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

P glycoprotein Antibody WB HeLa cells 22336-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 22336-1-AP (P glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

P glycoprotein Antibody IP human placenta tissue 22336-1-AP

IP Result of anti-P glycoprotein (IP:22336-1-AP, 4ug; Detection:22336-1-AP 1:300) with human placenta tissue lysate 4000ug.

P glycoprotein Antibody IHC human liver cancer tissue 22336-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP( P glycoprotein antibody at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P glycoprotein Antibody IHC human liver cancer tissue 22336-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP( P glycoprotein antibody at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse
Positive WB detected in:
HeLa cells
Positive IP detected in:
human placenta tissue
Positive IHC detected in:
human liver cancer tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
P glycoprotein fusion protein Ag17811
Full name:
ATP-binding cassette, sub-family B (MDR/TAP), member 1
Calculated molecular weight:
1280aa,142 kDa
Observed molecular weight:
130-150 kDa
GenBank accession number:
BC130424
Gene ID (NCBI):
5243
Gene symbol
ABCB1
Synonyms
ABCB1, CD243, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein 1

Background

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

P glycoprotein Rabbit Polyclonal antibody

Yu Siong (Verified Customer), 03-01-2019
Applications
Western Blot,
Primary Antibody Dilution
1:500
P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)

P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)

Cell/Tissue Type
Porcine brain endothelial cells
Lot Number
It worked perfectly for my porcine brain endothelial cells!

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