P glycoprotein Polyclonal antibody

P glycoprotein Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22336-1-AP

Synonyms

ABCB1, CD243, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein 1, P-gp



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22336-1-AP is P glycoprotein Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P glycoprotein fusion protein Ag17811
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 1
Calculated molecular weight 1280 aa, 142 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC130424
Gene symbol ABCB1
Gene ID (NCBI) 5243
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for P glycoprotein antibody 22336-1-APDownload protocol
IHC protocol for P glycoprotein antibody 22336-1-APDownload protocol
IP protocol for P glycoprotein antibody 22336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Molecules

Effect of Stereochemical Configuration on the Transport and Metabolism of Catechins from Green Tea across Caco-2 Monolayers.

Authors - Zeyi Ai
humanWB

Nat Commun

Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases.

Authors - Hui-Hui Guo
humanWB

Med Sci Monit

PMID: 31761897

Authors - Yangle Li
humanWB

Front Chem

Novel Quinoline Compound Derivatives of NSC23925 as Potent Reversal Agents Against P-Glycoprotein-Mediated Multidrug Resistance.

Authors - Xingping Quan
humanWB

Adv Healthc Mater

Thermoresponsive Supramolecular Chemotherapy by "V"-Shaped Armed β-Cyclodextrin Star Polymer to Overcome Drug Resistance.

Authors - Xiaoshan Fan
humanWB

Cell Death Dis

1,25-dihydroxyvitamin D3 signaling-induced decreases in IRX4 inhibits NANOG-mediated cancer stem-like properties and gefitinib resistance in NSCLC cells.

Authors - Zhirong Jia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yu Siong (Verified Customer) (03-01-2019)

It worked perfectly for my porcine brain endothelial cells!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Porcine brain endothelial cells
P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)