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P glycoprotein Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 22336-1-AP

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Con: 72 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

P glycoprotein Antibody WB HepG2 cells 22336-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 22336-1-AP( P glycoprotein antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

P glycoprotein Antibody IP human placenta tissue 22336-1-AP

IP Result of anti-P glycoprotein (IP:22336-1-AP, 4ug; Detection:22336-1-AP 1:300) with human placenta tissue lysate 4000ug.

P glycoprotein Antibody IHC human liver cancer tissue 22336-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 22336-1-AP( P glycoprotein antibody at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P glycoprotein Antibody IHC human liver cancer tissue 22336-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 22336-1-AP( P glycoprotein antibody at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse
Positive WB detected in:
HepG2 cells
Positive IP detected in:
human placenta tissue
Positive IHC detected in:
human liver cancer tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
P glycoprotein fusion protein Ag17811
Full name:
ATP-binding cassette, sub-family B (MDR/TAP), member 1
Calculated molecular weight:
1280aa,142 kDa
Observed molecular weight:
130-150 kDa
GenBank accession number:
BC130424
Gene ID (NCBI):
5243
Gene symbol
ABCB1
Synonyms
ABCB1, CD243, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein 1

Background

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer.


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