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  • Featured Product
  • KD/KO Validated

ACLY Polyclonal antibody

ACLY Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB,IP,IF,FC,IHC,CoIP,ELISA

Conjugate

Unconjugated

Cat no : 15421-1-AP

Synonyms

ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACLY Polyclonal antibody (15421-1-AP)

    WB analysis using 15421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ACLY Polyclonal antibody (15421-1-AP)

    WB analysis of A549 using 15421-1-AP

    WB result of ACLY antibody (15421-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACLY transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using ACLY Polyclonal antibody (15421-1-AP)

    WB analysis using 15421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ACLY antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACLY Polyclonal antibody (15421-1-AP)

    WB analysis using 15421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ACLY Polyclonal antibody (15421-1-AP)

    IP experiment of HeLa using 15421-1-AP

    IP result of anti-ACLY (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:8000) with HeLa cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACLY Polyclonal antibody (15421-1-AP)

    IHC staining of human prostate cancer using 15421-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACLY Polyclonal antibody (15421-1-AP)

    IHC staining of human prostate cancer using 15421-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACLY Polyclonal antibody (15421-1-AP)

    IF Staining of HepG2 using 15421-1-AP

    Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using ACLY Polyclonal antibody (15421-1-AP)

    FC experiment of HepG2 using 15421-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ACLY (15421-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inL02 cells, A549 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15421-1-AP targets ACLY in WB,IP,IF,FC,IHC,CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACLY fusion protein Ag7709
Full Name ATP citrate lyase
Calculated Molecular Weight 121 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC006195
Gene Symbol ACLY
Gene ID (NCBI) 47
RRIDAB_2223741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACLY antibody 15421-1-APDownload protocol
IHC protocol for ACLY antibody 15421-1-APDownload protocol
IF protocol for ACLY antibody 15421-1-APDownload protocol
IP protocol for ACLY antibody 15421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.

Authors - Steven Zhao
View Article
mouseWB,IF

Nature

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Authors - Philipp Mews
View Article
human,mouseWB

Cell

Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.

Authors - Xiaojing Liu
  • KO Validated
View Article
mouseWB

Cell Metab

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Authors - Peng Zhou
View Article
mouseWB

Mol Cell

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation

Authors - Sophie Trefely
  • KO Validated
View Article
humanWB

Mol Cell

Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.

Authors - Sharanya Sivanand
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Uthra (Verified Customer) (01-28-2021)

Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Liver
ACLY Antibody Immunohistochemistry validation (1:200 dilution) in Liver (Cat no:15421-1-AP)
FH

K (Verified Customer) (12-20-2020)

This antibody is working good for WB (1:1000) and IHC (1:200)

  • Applications: Western Blot, Immunohistochemistry
ACLY Antibody Western Blot,Immunohistochemistry validation ( dilution) in (Cat no:15421-1-AP)