Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, L02 cells, Jurkat cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15421-1-AP targets ACLY in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7709 Product name: Recombinant human ACLY protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 751-1101 aa of BC006195 Sequence: MFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM Predict reactive species |
| Full Name | ATP citrate lyase |
| Calculated Molecular Weight | 121 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC006195 |
| Gene Symbol | ACLY |
| Gene ID (NCBI) | 47 |
| RRID | AB_2223741 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53396 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ACLY antibody 15421-1-AP | Download protocol |
| IF protocol for ACLY antibody 15421-1-AP | Download protocol |
| IHC protocol for ACLY antibody 15421-1-AP | Download protocol |
| IP protocol for ACLY antibody 15421-1-AP | Download protocol |
| WB protocol for ACLY antibody 15421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ACLY (ATP-citrate lyase), catalog number 15421-1-AP, has 142 citations published in over 80 journals, including Nature, Cell, Cell Metabolism, Nature Communications, Molecular Cell, EMBO Journal, Science Advances, Cell Reports, and Oncogene. Key research fields include lipid metabolism, cancer biology, fatty liver disease, epigenetics, immunology, neurology, and cardiovascular disease. The antibody is predominantly used for Western blot, with additional applications in immunofluorescence, immunohistochemistry, and co-immunoprecipitation.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Focal adhesion kinase/Src family kinase axis-mediated tyrosine phosphorylation of metabolic enzymes facilitates tumor metastasis | ||
Cancer Cell Lipid oxidation reprogramming in cancer-associated fibroblasts enhances CD8(+) T cell cytotoxicity and therapeutic response. | ||
Reviews
What customers say
All three reviewers gave this antibody a perfect 5-star rating. Users report excellent performance across multiple applications: Western blotting (1:1000 dilution), immunofluorescence, and immunohistochemistry (1:200 dilution). One reviewer specifically highlighted its effectiveness for IHC on PFA-fixed liver tissue. Overall, customers find this ATP citrate lyase antibody reliable and well-performing for routine research use across different staining techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Soma (Verified Customer) (01-28-2026) | This antibody (ATP citrate lyase) works well for western blotting and Immunofluorescence staining.
|
Uthra (Verified Customer) (01-28-2021) | Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.
 |
K (Verified Customer) (12-20-2020) | This antibody is working good for WB (1:1000) and IHC (1:200)
 |
