Back

ATP citrate lyase Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 15421-1-AP

Featured Product KD/KO validated

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 26 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Special offer: Add control/tag antibody for $99
Load more
Tested Applications
Applications:
ELISA, FC, IF, IP, WB
Application not tested? Please see our Guarantee


Images See all

15421-1-AP;K-562 cells

K-562 cells were subjected to SDS PAGE followed by western blot with 15421-1-AP(ATP citrate lyase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

15421-1-AP;HepG2 cells

Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

15421-1-AP;K-562 cells

IP Result of anti-ATP citrate lyase (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:1000) with K-562 cells lysate 3200ug.

15421-1-AP;HepG2 cells

1X10^6 HepG2 cells were stained with 0.2ug ATP citrate lyase antibody (15421-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells
Positive IP detected in:
K-562 cells
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:10-1:100

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATP citrate lyase fusion protein Ag7709
Full name:
ATP citrate lyase
Calculated molecular weight:
121 kDa
Observed molecular weight:
110-120 kDa
GenBank accession number:
BC006195
Gene ID (NCBI):
47
Gene symbol
ACLY
Synonyms
ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP
Background

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma ,ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.


Back
to top