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ATP citrate lyase Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 15421-1-AP

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Con: 26 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, FC, ELISA
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Images See all

ATP citrate lyase Antibody WB L02 cells 15421-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ATP citrate lyase Antibody IF HepG2 cells 15421-1-AP

Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ATP citrate lyase Antibody IP K-562 cells 15421-1-AP

IP Result of anti-ATP citrate lyase (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:1000) with K-562 cells lysate 3200ug.

ATP citrate lyase Antibody FC HepG2 cells 15421-1-AP

1X10^6 HepG2 cells were stained with 0.2ug ATP citrate lyase antibody (15421-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
L02 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected in:
K-562 cells
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATP citrate lyase fusion protein Ag7709
Full name:
ATP citrate lyase
Calculated molecular weight:
121 kDa
Observed molecular weight:
110-120 kDa
GenBank accession number:
BC006195
Gene ID (NCBI):
47
Gene symbol
ACLY
Synonyms
ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Background

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma ,ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.


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