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  • KD/KO Validated

ACLY Polyclonal antibody

ACLY Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15421-1-AP


ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Tested Applications

Positive WB detected inL02 cells, HeLa cells, K-562 cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected inK-562 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15421-1-AP targets ACLY in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACLY fusion protein Ag7709
Full Name ATP citrate lyase
Calculated molecular weight 121 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006195
Gene symbol ACLY
Gene ID (NCBI) 47
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for ACLY antibody 15421-1-APDownload protocol
IF protocol for ACLY antibody 15421-1-APDownload protocol
IP protocol for ACLY antibody 15421-1-APDownload protocol
FC protocol for ACLY antibody 15421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Immunol

Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase.

Authors - Dmitry Namgaladze
  • KD Validated

Cell Rep

Adipocyte ACLY Facilitates Dietary Carbohydrate Handling to Maintain Metabolic Homeostasis in Females.

Authors - Sully Fernandez
  • KO Validated

J Exp Clin Cancer Res

ACLY facilitates colon cancer cell metastasis by CTNNB1.

Authors - Jun Wen
  • KO Validated

Cell Rep

Control of Adipocyte Thermogenesis and Lipogenesis through β3-Adrenergic and Thyroid Hormone Signal Integration.

Authors - Adilson Guilherme

Int J Mol Sci

Knockdown of PKM2 Suppresses Tumor Growth and Invasion in Lung Adenocarcinoma.

Authors - Hong Sun

Front Pharmacol

Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.

Authors - Nian Yang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Uthra (Verified Customer) (01-28-2021)

Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Liver
ACLY Antibody Immunohistochemistry validation (1:200 dilution) in Liver (Cat no:15421-1-AP)

K (Verified Customer) (12-20-2020)

This antibody is working good for WB (1:1000) and IHC (1:200)

  • Applications: Western Blot,Immunohistochemistry
ACLY Antibody Western Blot,Immunohistochemistry validation ( dilution) in (Cat no:15421-1-AP)