- Featured Product
- KD/KO Validated
ACLY Polyclonal antibody
ACLY Polyclonal Antibody for FC, IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, CoIP,ELISA
Conjugate
Unconjugated
Cat no : 15421-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, HeLa cells, K-562 cells, Jurkat cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | K-562 cells |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 23 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
15421-1-AP targets ACLY in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACLY fusion protein Ag7709 |
| Full Name | ATP citrate lyase |
| Calculated molecular weight | 121 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC006195 |
| Gene symbol | ACLY |
| Gene ID (NCBI) | 47 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACLY antibody 15421-1-AP | Download protocol |
| IF protocol for ACLY antibody 15421-1-AP | Download protocol |
| IP protocol for ACLY antibody 15421-1-AP | Download protocol |
| FC protocol for ACLY antibody 15421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase.
| ||
Cell Rep Adipocyte ACLY Facilitates Dietary Carbohydrate Handling to Maintain Metabolic Homeostasis in Females.
| ||
J Exp Clin Cancer Res ACLY facilitates colon cancer cell metastasis by CTNNB1.
| ||
Cell Rep Control of Adipocyte Thermogenesis and Lipogenesis through β3-Adrenergic and Thyroid Hormone Signal Integration. | ||
Int J Mol Sci Knockdown of PKM2 Suppresses Tumor Growth and Invasion in Lung Adenocarcinoma. | ||
Front Pharmacol Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Uthra (Verified Customer) (01-28-2021) | Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.
 |
FH K (Verified Customer) (12-20-2020) | This antibody is working good for WB (1:1000) and IHC (1:200)
 |
