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CoraLite®488-conjugated ACLY Polyclonal antibody

ACLY Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-15421

Synonyms

ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP



Tested Applications

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 15421-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-15421 targets ACLY in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACLY fusion protein Ag7709
Full Name ATP citrate lyase
Calculated molecular weight 121 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006195
Gene symbol ACLY
Gene ID (NCBI) 47
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for CL488 ACLY antibody CL488-15421Download protocol
Standard Protocols
Click here to view our Standard Protocols