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ACTL6A Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 10341-1-AP

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Con: 29 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

ACTL6A Antibody WB K-562 cells 10341-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 10341-1-AP(ACTL6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ACTL6A Antibody IHC human ovary tissue 10341-1-AP

Immunohistochemical of paraffin-embedded human ovary using 10341-1-AP(ACTL6A antibody) at dilution of 1:50 (under 10x lens)

ACTL6A Antibody IHC human ovary tissue 10341-1-AP

Immunohistochemical of paraffin-embedded human ovary using 10341-1-AP(ACTL6A antibody) at dilution of 1:50 (under 40x lens)

ACTL6A Antibody IP HeLa cells 10341-1-AP

IP Result of anti-ACTL6A (IP:10341-1-AP, 4ug; Detection:10341-1-AP 1:500) with HeLa cells lysate 1200ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells, HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACTL6A fusion protein Ag0323
Full name:
actin-like 6A
Calculated molecular weight:
47 kDa
Observed molecular weight:
45-51 kDa
GenBank accession number:
BC001391
Gene ID (NCBI):
86
Gene symbol
ACTL6A
Synonyms
actin like 6A, Actin like protein 6A, Actin related protein Baf53a, ACTL6, ACTL6A, Arp4, ARPN BETA, ArpNbeta, BAF53, BAF53A, BRG1 associated factor 53A, INO80 complex subunit K, INO80K

Background

Actin-like 6A (ACTL6A, synonyms: ACTL6, BAF53A, MGC5382) is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A is a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix.


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