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  • KD/KO Validated

CCP1 Polyclonal antibody

CCP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14067-1-AP

Synonyms

AGTPBP1, ATP/GTP binding protein 1, CCP1, Cytosolic carboxypeptidase 1, KIAA1035, NNA1



Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14067-1-AP targets CCP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCP1 fusion protein Ag5122
Full Name ATP/GTP binding protein 1
Calculated Molecular Weight 138 kDa
Observed Molecular Weight 138 kDa
GenBank Accession NumberBC060815
Gene Symbol AGTPBP1
Gene ID (NCBI) 23287
RRIDAB_2225559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.

Protocols

Product Specific Protocols
WB protocol for CCP1 antibody 14067-1-APDownload protocol
IHC protocol for CCP1 antibody 14067-1-APDownload protocol
IF protocol for CCP1 antibody 14067-1-APDownload protocol
IP protocol for CCP1 antibody 14067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis.

Authors - Carla G Silva
mouseWB

Nat Commun

Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.

Authors - Buqing Ye
  • KO Validated
human,mouseWB,IF

EMBO J

Loss of tubulin deglutamylase CCP1 causes infantile-onset neurodegeneration.

Authors - Vandana Shashi
mouseWB

Nat Commun

AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.

Authors - Albert Herms
ratWB

Proc Natl Acad Sci U S A

Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy.

Authors - Maria Elena Pero
  • KO Validated
human,mouseWB,IP

J Cell Biol

CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice.

Authors - Stephen Gilmore-Hall