15 Lipoxygenase 2 Polyclonal antibody

15 Lipoxygenase 2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13073-1-AP

Synonyms

15 Lipoxygenase 2, 15 LOX 2, 15 LOX B, ALOX15B, ALOX8, Arachidonate 15 lipoxygenase B



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HL-60 cells, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13073-1-AP targets 15 Lipoxygenase 2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 15 Lipoxygenase 2 fusion protein Ag3741
Full Name arachidonate 15-lipoxygenase, type B
Calculated molecular weight 676 aa, 76 kDa
Observed molecular weight 67-76 kDa
GenBank accession numberBC035217
Gene symbol ALOX15B
Gene ID (NCBI) 247
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.

Protocols

Product Specific Protocols
WB protocol for 15 Lipoxygenase 2 antibody 13073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Chem Neuroanat

Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.

Authors - Huangao Zhou