ALPL Polyclonal antibody

ALPL Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 11187-1-AP

Synonyms

Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, ALPL, BLAP, Bone Alkaline Phosphatase, NBAP



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

11187-1-AP targets ALPL in WB, IP, IHC, FC, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALPL fusion protein Ag1664
Full Name alkaline phosphatase, liver/bone/kidney
Calculated molecular weight 57 kDa
Observed molecular weight 80 kDa, 66 kDa
GenBank accession numberBC021289
Gene symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.

Protocols

Product Specific Protocols
WB protocol for ALPL antibody 11187-1-APDownload protocol
IHC protocol for ALPL antibody 11187-1-APDownload protocol
IF protocol for ALPL antibody 11187-1-APDownload protocol
IP protocol for ALPL antibody 11187-1-APDownload protocol
FC protocol for ALPL antibody 11187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Biol Int

Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA-TAZ axis.

Authors - Yunhe Xu
mouseIHC

Anat Cell Biol

Col1a1-cre mediated activation of β-catenin leads to aberrant dento-alveolar complex formation.

Authors - Kim Tak-Heun TH
mouseWB

Cell Death Dis

eIF2α signaling regulates autophagy of osteoblasts and the development of osteoclasts in OVX mice.

Authors - Jie Li
pigWB

J Huazhong Univ Sci Technolog Med Sci

Role of Wnt/β-catenin signaling pathway in the mechanism of calcification of aortic valve.

Authors - Gang-jian Gu
pigIHC

Dent Mater J

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer.

Authors - Sun Ping P
humanWB

Cells Tissues Organs

Effect of MicroRNA-20a on Osteogenic Differentiation of Human Adipose Tissue-Derived Stem Cells.

Authors - Tao Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Smear band but with the right product size on western blot. Immunohistochemical staining showed desired results in control versus knockout mouse model

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse colon tissue
FH

Jingwen (Verified Customer) (01-17-2020)

This antibody worked very well and showed the specific band around 70kDa in western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart section