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APOBEC3G Monoclonal antibody

APOBEC3G Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5G10A8

Cat no : 60100-1-Ig

Synonyms

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019



Tested Applications

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

60100-1-Ig targets APOBEC3G in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen APOBEC3G fusion protein Ag7770
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC024268
Gene symbol APOBEC3G
Gene ID (NCBI) 60489
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Publications

SpeciesApplicationTitle
humanIHC

Int J Clin Exp Med

APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.

Authors - Huanrong Lan
humanWB

Sci Rep

Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.

Authors - Ruidong Chen