ARF5-Specific Polyclonal antibody

ARF5-Specific Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20227-1-AP

Synonyms

ADP ribosylation factor 5, ARF5



Tested Applications

Positive WB detected inHepG2 cells, mouse colon tissue, mouse liver tissue, HeLa cells, mouse kidney tissue, mouse brain tissue, COLO 320 cells, human testis tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20227-1-AP targets ARF5-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ADP-ribosylation factor 5
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberNM_001662
Gene Symbol ARF5
Gene ID (NCBI) 381
RRIDAB_10639517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). Their function are not fully described.The antibody is specific to ARF5.

Protocols

Product Specific Protocols
WB protocol for ARF5-Specific antibody 20227-1-APDownload protocol
IF protocol for ARF5-Specific antibody 20227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

PeerJ

Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection.

Authors - Ruichao Li
mouseWB

Cell Rep

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis

Authors - Rakhee Bajaj
  • KD Validated