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ATG7 Antibody 1 Publications

Mouse Monoclonal| Catalog number: 67341-1-Ig |CloneNo.: 2B5A10

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

ATG7 Antibody WB Jurkat cells 67341-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

ATG7 Antibody IHC human cervical cancer tissue 67341-1-Ig

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ATG7 Antibody IF HeLa cells 67341-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Human, Mouse, Rat, Pig
Positive WB detected in:
Jurkat cells, HEK-293 cells, pig spleen tissue, HepG2 cells, THP-1 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected in:
human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:500-1:2000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATG7 fusion protein Ag29172
Full name:
ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated molecular weight:
78 kDa
Observed molecular weight:
68-78 kDa
GenBank accession number:
BC000091
Gene ID (NCBI):
10533
Gene symbol
ATG7
Synonyms
APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

Background

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.


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