|Positive WB detected in||Jurkat cells, HEK-293 cells, pig spleen tissue, HepG2 cells, THP-1 cells, NIH/3T3 cells, HSC-T6 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
67341-1-Ig targets ATG7 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
|Tested Reactivity||Human, Mouse, Rat, Pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||ATG7 fusion protein Ag29172|
|Full Name||ATG7 autophagy related 7 homolog (S. cerevisiae)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||68-78 kDa|
|GenBank accession number||BC000091|
|Gene ID (NCBI)||10533|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
Biol Pharm Bull
Mol Med Rep
Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy.
SHANK3 Co-ordinately Regulates Autophagy and Apoptosis in Myocardial Infarction.
Appl Microbiol Biotechnol
Latcripin-7A, derivative of Lentinula edodes C91-3, reduces migration and induces apoptosis, autophagy, and cell cycle arrest at G1 phase in breast cancer cells.
Cardamonin Reduces Acetaminophen-Induced Acute Liver Injury in Mice via Activating Autophagy and NFE2L2 Signaling.