Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 67341-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 67341-1-Ig
LNCaP cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of HeLa using 67341-1-Ig
WB result of ATG7 antibody (67341-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HeLa cells.
WB analysis of HepG2 using 67341-1-Ig
WB result of ATG7 antibody (67341-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells.
WB analysis of 4T1 using 67341-1-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig spleen using 67341-1-Ig
pig spleen tissue were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 67341-1-Ig
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 67341-1-Ig
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 67341-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of HeLa using 67341-1-Ig
1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATG7 (67341-1-Ig, Clone:2B5A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ATG7 Monoclonal antibody

ATG7 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 67341-1-Ig

Clone No.2B5A10

Publications(37)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L, APG7 LIKE

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (37)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	LNCaP cells, Jurkat cells, pig spleen tissue, HepG2 cells, 4T1 cells, HeLa cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 34 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

67341-1-Ig targets ATG7 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, canine
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	ATG7 fusion protein Ag29172 Predict reactive species
Full Name	ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated Molecular Weight	78 kDa
Observed Molecular Weight	68-78 kDa
GenBank Accession Number	BC000091
Gene Symbol	ATG7
Gene ID (NCBI)	10533
RRID	AB_2882599
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O95352
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protocols

Product Specific Protocols
WB protocol for ATG7 antibody 67341-1-Ig	Download protocol
IHC protocol for ATG7 antibody 67341-1-Ig	Download protocol
IF protocol for ATG7 antibody 67341-1-Ig	Download protocol
FC protocol for ATG7 antibody 67341-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer PMID: 32727463 Authors - Cefan Zhou View Article
human	WB	Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy Authors - Lihao Jin View Article
human	WB	Cancers (Basel) Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators. Authors - David P Dannheisig View Article
mouse	WB	J Cell Physiol Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway Authors - Jingru Guo View Article
mouse	WB	Stem Cell Res Ther Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling. Authors - Jie Zhou View Article
human	WB	Transl Oncol METTL3 methylated KIF15 promotes nasopharyngeal carcinoma progression and radiation resistance by blocking ATG7-mediated autophagy through the activation of STAT3 pathway Authors - Siwei Li View Article