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CoraLite® Plus 488-conjugated ATG9A Monoclonal antibody

ATG9A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D12E11

Cat no : CL488-67096

Synonyms

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ATG9A Monoclonal ant (CL488-67096)

    IF Staining of HeLa using CL488-67096

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated ATG9A antibody (CL488-67096, Clone: 1D12E11 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ATG9A Monoclonal ant (CL488-67096)

    IF Staining of HepG2 using CL488-67096

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-67096 (ATG9A antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67096 targets ATG9A in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG9A fusion protein Ag24278
Full Name ATG9 autophagy related 9 homolog A (S. cerevisiae)
Calculated Molecular Weight 837 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC065534
Gene Symbol ATG9A
Gene ID (NCBI) 79065
RRIDAB_2883397
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ATG9A antibody CL488-67096Download protocol
Standard Protocols
Click here to view our Standard Protocols