ATP1B2 Antibody

ATP1B2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22338-1-AP

Synonyms

AMOG, ATP1B2, ATPase Na+/K+ beta 2



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, C2C12 cell, C6 cells, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 22338-1-AP immunogen is Fusion Protein ATP1B2 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1B2 fusion protein Ag17818
Full Name ATPase, Na+/K+ transporting, beta 2 polypeptide
Calculated molecular weight 290aa,33 kDa
Observed molecular weight 45-65 kDa
GenBank accession number BC126175
Gene symbol ATP1B2
Gene ID (NCBI) 482
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1B2 is the β2 subunit of Na+/K+-ATPase which is an essential membrane-bound enzyme responsible for the transport of Na+ and K+ in most eukaryotic cells. ATP1B2 is also called the adhesion molecule on glia (AMOG) and it is highly expressed in normal glia. It is a heavily glycosylated protein that plays a role in cellular adhesion in the CNS. Recently differential expression of ATP1B2 has been found in some glioneuronal tumors (23887941, 19371356). This antibody recognizes the endogenous ATP1B2 protein in human brain. The bands between 45 kDa and 65kDa represent the glycosylated forms of ATP1B2 in different levels (8918259).

Protocols

Product Specific Protocols
WB protocol for ATP1B2 antibody 22338-1-APDownload protocol
IHC protocol for ATP1B2 antibody 22338-1-APDownload protocol
IP protocol for ATP1B2 antibody 22338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

J Neurosci

ARL13B, a Joubert Syndrome-associated protein, is critical for retinogenesis and elaboration of mouse photoreceptor outer segments.

Authors - Tanya L Dilan
mouseIF

Cell Mol Gastroenterol Hepatol

Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells.

Authors - Yuli Wang
humanWB

Eur J Appl Physiol

Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.

Authors - Muath M Altarawneh
ratWB

Redox Biol

Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration.

Authors - Xiaoying Luo