ATP5A1 Antibody 15 Publications

Rabbit Polyclonal| Catalog number: 14676-1-AP

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Freight/Packing

Con: 24 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
multi-cells/tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, PC-3 cells, rat heart tissue, Y79 cells

Positive IHC detected in:
human liver tissue, human kidney tissue

Positive IF detected in:
HepG2 cells, Hela cells, HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle

Calculated molecular weight:
60 kDa

Observed molecular weight:
50-55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ATP5A1

Synonyms
ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, MOM2, OMR, ORM
Background

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.


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