|Positive WB detected in||HeLa cells, rat heart tissue|
|Positive IHC detected in||human liver tissue, human kidney tissue, human brain tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14676-1-AP targets ATP5A1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP5A1 fusion protein Ag6385|
|Full Name||ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC064562|
|Gene ID (NCBI)||498|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo-containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.
TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.
Biochim Biophys Acta Gene Regul Mech
The 3'UTR of human MAVS mRNA contains multiple regulatory elements for the control of protein expression and subcellular localization.
Int J Mol Sci
Taurine Inhibits Glucocorticoid-Induced Bone Mitochondrial Injury, Preventing Osteonecrosis in Rabbits and Cultured Osteocytes.
A Regulation Loop between YAP and NR4A1 Balances Cell Proliferation and Apoptosis.
Cell Death Dis
A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.
Mol Med Rep
Protein profiling and functional analysis of liver mitochondria from rats with nonalcoholic steatohepatitis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
XB (Verified Customer) (10-29-2020)
It probes ATP5A1 clearly with a right MW 55 kD.
Siting (Verified Customer) (09-04-2020)