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  • Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours. <Br>We recommend using 12% separating gel or 8%-18% gradient gel.

    Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.
    We recommend using 12% separating gel or 8%-18% gradient gel.

  • Blue Native PAGE showing oxidative phosphorylation (OXPHOS) complexes and super complexes in mitochondria. HepG2 cell lysates were subjected to BN PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:5000 and antibodies to each complex incubated at room temperature for 1.5 hours.

    Blue Native PAGE showing oxidative phosphorylation (OXPHOS) complexes and super complexes in mitochondria. HepG2 cell lysates were subjected to BN PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:5000 and antibodies to each complex incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

OXPHOS Cocktail (Human recommended)

Cat No. PK30006

Publications

20

Review Product

Conjugate

Unconjugated

Applications

WB

Reactivity

Human

Host

Rabbit

Size: 

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Freight/Packing: -

Quantity

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Product Information

OXPHOS (oxidative phosphorylation) requires the transport of electrons to molecular oxygen by the mitochondrial respiratory chain which involves four multi-subunit complexes known as complexes I, II, III, and IV (CI, CII, CIII and CIV) and two mobile electron carriers, coenzyme Q (CoQ) and cytochrome c. The respiratory chain generates a transmembrane proton gradient that is channeled by complex V (also known as ATP synthase, CV) to synthesize ATP.

    This OXPHOS kit contains five Polyclonal antibodies. The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from  human. Individual proteins that need to be tested can be purchased according to the corresponding catalog number.



Cat.No            Name            

Complex                

MW            Size            
Antibodies            14676-1-AP            ATP5A1            Complexes Ⅴ            50-55 kDa            100ul
            
21705-1-AP            UQCRC1            Complexes Ⅲ            45 kDa            
10620-1-AP            SDHB            Complexes Ⅱ            32 kDa            
55070-1-AP            MTCO2            Complexes Ⅳ            23-25 kDa            
14794-1-AP            NDUFB8            ComplexesⅠ            19-22 kDa            


Storage

Store at -20℃. Stable for one year after shipment.

Cited in Article as

PK30006, OXPHOS Cocktail (Human recommended), Proteintech, IL, USA

Publications

ApplicationTitle

Biochem Pharmacol

The mitochondrial protease ClpP is a promising target for multiple myeloma treatment

Authors - Xiang Liu
View Article

J Extracell Vesicles

S100A9 Aggravates Cardiac Fibrosis by TLR4/PGC-1α Mediated Macrophage-Myofibroblast Crosstalk.

Authors - Yiting Zhao
View Article

Cell Signal

Erucic acid aggravates cardiac fibrosis after myocardial infarction by CD36-mediated metabolic reprogramming with impaired mitochondrial aerobic oxidation.

Authors - Meng Zhang
View Article

Ann Hematol

Evaluated NSUN3 in reticulocytes from HbH-CS disease that reflects cellular stress in erythroblasts

Authors - Haodong Liu
View Article

Front Pharmacol

Piroxicam accelerates diabetic foot ulcer healing via ERα-dependent mitochondrial protection and oxidative stress relief.

Authors - Qing-Qing Liao
View Article
WB

Cell Death Discov

Lysophosphatidylcholine acyltransferase 1 promotes head and neck squamous cell carcinoma progression by enhancing COX17-dependent oxidative phosphorylation.

Authors - Yuanyang Zhao
View Article
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