|Positive WB detected in||mouse liver tissue, mouse heart tissue, mouse skeletal muscle tissue|
|Positive IHC detected in||human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human skin tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15999-1-AP targets ATP5F1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP5F1 fusion protein Ag8571|
|Full Name||ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1|
|Calculated molecular weight||256 aa, 29 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC005366|
|Gene ID (NCBI)||515|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.
J Agric Food Chem
A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.
J Cell Biochem
F1 FO ATP Synthase Is Expressed at the Surface of Embryonic Rat Heart-Derived H9c2 Cells and Is Affected by Cardiac-Like Differentiation.
Heat Stress Impairs the Physiological Responses and Regulates Genes Coding for Extracellular Exosomal Proteins in Rat.
Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.
J Biol Chem
An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
siting (Verified Customer) (07-13-2020)
very good signal
Yan (Verified Customer) (01-28-2020)
It worked very well with mouse heart tissue for western blot.