ATP5F1 Polyclonal antibody

ATP5F1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15999-1-AP

Synonyms

ATP5F1, ATPase subunit b, PIG47



Tested Applications

Positive WB detected inmouse liver tissue, mouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman liver tissue, human brain tissue, human heart tissue, human kidney tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15999-1-AP targets ATP5F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5F1 fusion protein Ag8571
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
Calculated molecular weight 256 aa, 29 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC005366
Gene symbol ATP5F1
Gene ID (NCBI) 515
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

Protocols

Product Specific Protocols
WB protocol for ATP5F1 antibody 15999-1-APDownload protocol
IHC protocol for ATP5F1 antibody 15999-1-APDownload protocol
IF protocol for ATP5F1 antibody 15999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.

Authors - Zhang Jun J
mouseWB

J Agric Food Chem

A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.

Authors - Cheng Liu
ratIF

J Cell Biochem

F1 FO ATP Synthase Is Expressed at the Surface of Embryonic Rat Heart-Derived H9c2 Cells and Is Affected by Cardiac-Like Differentiation.

Authors - Marina Comelli
ratWB

Genes (Basel)

Heat Stress Impairs the Physiological Responses and Regulates Genes Coding for Extracellular Exosomal Proteins in Rat.

Authors - Jinhuan Dou
humanWB

FEBS Lett

Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.

Authors - Makoto Fujikawa
humanWB

Cancer Sci

Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.

Authors - Lianqin Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

siting (Verified Customer) (07-13-2020)

very good signal

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse heart cells
ATP5F1 Antibody Western Blot, validation (1:1000 dilution) in mouse heart cells (Cat no:15999-1-AP)
FH

Yan (Verified Customer) (01-28-2020)

It worked very well with mouse heart tissue for western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue extract