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BACH1 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 14018-1-AP

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

BACH1 Antibody WB HEK-293 cells 14018-1-AP

WB result of BACH1 antibody (14018-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells

BACH1 Antibody WB HEK-293 cells 14018-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14018-1-AP(BACH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

BACH1 Antibody IHC human breast hyperplasia tissue 14018-1-AP

Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14018-1-AP( BACH1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

BACH1 Antibody IHC human breast hyperplasia tissue 14018-1-AP

Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14018-1-AP( BACH1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, HeLa cells, MCF-7 cells, Raji cells
Positive IHC detected in:
human breast hyperplasia tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
BACH1 fusion protein Ag5128
Full name:
BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated molecular weight:
82 kDa
Observed molecular weight:
100-110 kDa, 82 kDa
GenBank accession number:
BC063307
Gene ID (NCBI):
571
Gene symbol
BACH1
Synonyms
BACH 1, BACH1, BTB and CNC homolog 1, HA2303

Background

BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle 

regulation in homeostasis and in cancer in disease.


What is the molecular weight of BACH1?

Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor 

family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs 

anomalously at 100-110 kDa in SDS-PAGE.


What is the cellular localization of BACH1?

As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a 

heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the 

Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by 

heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to 

microtubules (PMID: 15809329).


What is the function of BACH1?

BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme 

oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved 

in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor 

(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a 

role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6 

(CDK6) and BCL2-like 11 (BCL2L11).


What is the role of BACH1 in disease?

BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function 

of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis, 

influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787

892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).



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