Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, Raji cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human cervical cancer tissue, human breast hyperplasia tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 39 publications below |
| IHC | See 14 publications below |
| IF | See 15 publications below |
| FC | See 1 publications below |
| ChIP | See 4 publications below |
Product Information
14018-1-AP targets BACH1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BACH1 fusion protein Ag5128 Predict reactive species |
| Full Name | BTB and CNC homology 1, basic leucine zipper transcription factor 1 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC063307 |
| Gene Symbol | BACH1 |
| Gene ID (NCBI) | 571 |
| ENSEMBL Gene ID | ENSG00000156273 |
| RRID | AB_2274498 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14867 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle
regulation in homeostasis and in cancer in disease.
What is the molecular weight of BACH1?
Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor
family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs
anomalously at 100-110 kDa in SDS-PAGE.
What is the cellular localization of BACH1?
As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a
heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the
Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by
heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to
microtubules (PMID: 15809329).
What is the function of BACH1?
BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme
oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved
in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor
(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a
role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6
(CDK6) and BCL2-like 11 (BCL2L11).
What is the role of BACH1 in disease?
BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function
of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis,
influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787
892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BACH1 antibody 14018-1-AP | Download protocol |
| IHC protocol for BACH1 antibody 14018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. | ||
Autophagy MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis | ||
J Exp Clin Cancer Res Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancer | ||
J Nanobiotechnology Eriodictyol-cisplatin coated nanomedicine synergistically promote osteosarcoma cells ferroptosis and chemosensitivity | ||
Cancer Lett PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer. | ||
