|Positive WB detected in||HEK-293 cells, HeLa cells, MCF-7 cells, Raji cells|
|Positive IHC detected in||human breast hyperplasia tissue, human breast cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14018-1-AP targets BACH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BACH1 fusion protein Ag5128|
|Full Name||BTB and CNC homology 1, basic leucine zipper transcription factor 1|
|Calculated molecular weight||82 kDa|
|Observed molecular weight||100-110 kDa, 82 kDa|
|GenBank accession number||BC063307|
|Gene ID (NCBI)||571|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle
regulation in homeostasis and in cancer in disease.
What is the molecular weight of BACH1?
Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor
family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs
anomalously at 100-110 kDa in SDS-PAGE.
What is the cellular localization of BACH1?
As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a
heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the
Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by
heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to
microtubules (PMID: 15809329).
What is the function of BACH1?
BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme
oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved
in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor
(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a
role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6
(CDK6) and BCL2-like 11 (BCL2L11).
What is the role of BACH1 in disease?
BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function
of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis,
influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787
892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).
S-1-Propenylcysteine augments BACH1 degradation and heme oxygenase 1 expression in a nitric oxide-dependent manner in endothelial cells.
Biol Pharm Bull
Astragaloside IV regulates the PI3K/Akt/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation.
Int J Cell Biol
Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway.
Dynamic changes in Bach1 expression in the kidney of rhabdomyolysis-associated acute kidney injury.
The electrophilic character of quinones is essential for the suppression of Bach1.
Tetrachlorobenzoquinone induces Nrf2 activation via rapid Bach1 nuclear export/ubiquitination and JNK-P62 signaling.