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BACH1 Polyclonal antibody

BACH1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14018-1-AP

Synonyms

BACH 1, BACH1, BTB and CNC homolog 1, HA2303



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14018-1-AP targets BACH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BACH1 fusion protein Ag5128
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated molecular weight 82 kDa
Observed molecular weight 100-110 kDa, 82 kDa
GenBank accession numberBC063307
Gene symbol BACH1
Gene ID (NCBI) 571
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle 

regulation in homeostasis and in cancer in disease.


What is the molecular weight of BACH1?

Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor 

family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs 

anomalously at 100-110 kDa in SDS-PAGE.


What is the cellular localization of BACH1?

As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a 

heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the 

Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by 

heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to 

microtubules (PMID: 15809329).


What is the function of BACH1?

BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme 

oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved 

in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor 

(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a 

role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6 

(CDK6) and BCL2-like 11 (BCL2L11).


What is the role of BACH1 in disease?

BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function 

of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis, 

influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787

892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).


Protocols

Product Specific Protocols
WB protocol for BACH1 antibody 14018-1-APDownload protocol
IHC protocol for BACH1 antibody 14018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nitric Oxide

S-1-Propenylcysteine augments BACH1 degradation and heme oxygenase 1 expression in a nitric oxide-dependent manner in endothelial cells.

Authors - Tadamitsu Tsuneyoshi
ratWB

Biol Pharm Bull

Astragaloside IV regulates the PI3K/Akt/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation.

Authors - Ping Yang
humanWB

Int J Cell Biol

Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway.

Authors - Kwok Simon C M SC
ratWB

PLoS One

Dynamic changes in Bach1 expression in the kidney of rhabdomyolysis-associated acute kidney injury.

Authors - Masakazu Yamaoka
humanWB,IF

Toxicology

The electrophilic character of quinones is essential for the suppression of Bach1.

Authors - Chuanyang Su
humanWB,IF

Toxicology

Tetrachlorobenzoquinone induces Nrf2 activation via rapid Bach1 nuclear export/ubiquitination and JNK-P62 signaling.

Authors - Chuanyang Su
  • KD Validated