• Featured Product
  • KD/KO Validated

TGFBI / BIGH3 Polyclonal antibody

TGFBI / BIGH3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Neutralization, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10188-1-AP

Synonyms

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI



Tested Applications

Positive WB detected inmouse eye tissue, HeLa cells, human kidney tissue, mouse liver tissue, Y79 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10188-1-AP targets TGFBI / BIGH3 in WB, Neutralization, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGFBI / BIGH3 fusion protein Ag0241
Full Name transforming growth factor, beta-induced, 68kDa
Calculated molecular weight 683 aa, 75 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC000097
Gene symbol TGFBI
Gene ID (NCBI) 7045
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Protocols

Product Specific Protocols
WB protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
IHC protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
IF protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
IP protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
FC protocol for TGFBI / BIGH3 antibody 10188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Onco Targets Ther

Collagen stiffness promoted non-muscle-invasive bladder cancer progression to muscle-invasive bladder cancer.

Authors - Huier Zhu
humanWB

Mol Ther Nucleic Acids

PPAR Gamma-Regulated MicroRNA 199a-5p Underlies Bone Marrow Adiposity in Aplastic Anemia.

Authors - Xianning Zhang
  • KD Validated
humanWB,IHC

Cancer Manag Res

Secreted TGF-beta-induced protein promotes aggressive progression in bladder cancer cells.

Authors - Jun Zou
  • KD Validated
humanWB,IHC

Urol Oncol

Identification and validation of TGFBI as a promising prognosis marker of clear cell renal cell carcinoma.

Authors - Souhil Lebdai
humanIHC

Int Med Case Rep J

Collagen fiber changes related to keratoconus with secondary corneal amyloidosis.

Authors - Kaoru Araki-Sasaki
humanNeutralization

Cell Death Dis

Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and tenascin C.

Authors - Anna Mary Steitz