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TGFBI / BIGH3 Antibody 35 Publications

Rabbit Polyclonal| Catalog number: 10188-1-AP

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Con: 34 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

TGFBI / BIGH3 Antibody WB mouse eye tissue 10188-1-AP

mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

TGFBI / BIGH3 Antibody IHC human liver cancer tissue 10188-1-AP

Immunohistochemical of paraffin-embedded human liver cancer using 10188-1-AP(TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens)

TGFBI / BIGH3 Antibody IHC human kidney tissue 10188-1-AP

Immunohistochemical of paraffin-embedded human kidney using 10188-1-AP(TGFBI / BIGH3 antibody) at dilution of 1:100 (under 10x lens)

TGFBI / BIGH3 Antibody IHC human kidney tissue 10188-1-AP

Immunohistochemical of paraffin-embedded human kidney using 10188-1-AP(TGFBI / BIGH3 antibody) at dilution of 1:100 (under 40x lens)

TGFBI / BIGH3 Antibody IF HeLa cells 10188-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 10188-1-AP(TGFBI / BIGH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TGFBI / BIGH3 Antibody FC HeLa cells 10188-1-AP

1X10^6 HeLa cells were stained with 0.2ug TGFBI / BIGH3 antibody (10188-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

TGFBI / BIGH3 Antibody IP HeLa cells 10188-1-AP

IP Result of anti-TGFBI / BIGH3 (IP:10188-1-AP, 3ug; Detection:10188-1-AP 1:300) with HeLa cells lysate 1000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse eye tissue, HeLa cells, human kidney tissue, mouse liver tissue, Y79 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human kidney tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TGFBI / BIGH3 fusion protein Ag0241
Full name:
transforming growth factor, beta-induced, 68kDa
Calculated molecular weight:
683aa,75 kDa
Observed molecular weight:
64 kDa
GenBank accession number:
BC000097
Gene ID (NCBI):
7045
Gene symbol
TGFBI
Synonyms
Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI

Background

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).


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