Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10188-1-AP
Mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10188-1-AP
WB result of TGFBI / BIGH3 antibody (10188-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBI / BIGH3 transfected HeLa cells.
WB analysis of mouse eye using 10188-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 10188-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10188-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 10188-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 10188-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10188-1-AP
IP result of anti-TGFBI / BIGH3 (IP:10188-1-AP, 3ug; Detection:10188-1-AP 1:300) with HeLa cells lysate 1000ug.
IHC staining of human kidney using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse eye using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 10188-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 10188-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells, untreated (left) or TGF-β-treated (right), using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of Y79 using 10188-1-AP
Immunofluorescent analysis of (4% PFA) fixed Y79 cells using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of Y79 using 10188-1-AP
1X10^6 Y79 cells were intracellularly stained with 0.4 ug Anti-Human TGFBI / BIGH3 (10188-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

TGFBI/BIGH3 Polyclonal antibody

Name: TGFBI/BIGH3 Polyclonal antibody
Brand: Proteintech
SKU: 10188-1-AP
Rating: 3.00 (1 reviews)

TGFBI/BIGH3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10188-1-AP

Publications(71)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Neutralization, ELISA, Cell treatment

Synonyms

TGFBI, TGFBI / BIGH3, RGD-containing collagen-associated protein, RGD-CAP, Kerato-epithelin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (71)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse eye tissue, mouse liver tissue, human kidney tissue, Y79 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue, human liver cancer tissue, mouse eye tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	TGF beta 1 treated A549 cells, Y79 cells
Positive FC (Intra) detected in	Y79 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 41 publications below
IHC	See 36 publications below
IF	See 16 publications below
IP	See 4 publications below
FC	See 1 publications below

Product Information

10188-1-AP targets TGFBI/BIGH3 in WB, IHC, IF/ICC, FC (Intra), IP, Neutralization, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TGFBI/BIGH3 fusion protein Ag0241 Predict reactive species
Full Name	transforming growth factor, beta-induced, 68kDa
Calculated Molecular Weight	683 aa, 75 kDa
Observed Molecular Weight	64 kDa
GenBank Accession Number	BC000097
Gene Symbol	TGFBI
Gene ID (NCBI)	7045
ENSEMBL Gene ID	ENSG00000120708
RRID	AB_2202311
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15582
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Protocols

Product Specific Protocols
WB protocol for TGFBI/BIGH3 antibody 10188-1-AP	Download protocol
IHC protocol for TGFBI/BIGH3 antibody 10188-1-AP	Download protocol
IF protocol for TGFBI/BIGH3 antibody 10188-1-AP	Download protocol
IP protocol for TGFBI/BIGH3 antibody 10188-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IF	Nat Cell Biol Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets. Authors - Qin Zhang View Article
human	WB	Nat Protoc Comprehensive isolation of extracellular vesicles and nanoparticles Authors - Qin Zhang View Article
human	IP	Sci Adv Acetylated K676 TGFBIp as a severity diagnostic blood biomarker for SARS-CoV-2 pneumonia. Authors - Hee Ho Park View Article
mouse	WB,IHC	Genes Dev Extracellular matrix protein betaig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation. Authors - Ma Chaoyu C KD Validated View Article
human	IHC,IF	Theranostics Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2 Authors - Zirong Chen View Article
human	WB,IP	Biomaterials Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis. Authors - Wonhwa Lee View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Carmelo (Verified Customer) (04-25-2022)

I tested the abs 1:1000 ON at 4C on 40ug spinal cord protein lysates. unfortunately I was not able to distinguish a proper band at the expected size. I will run further optimisation in the incoming weeks.