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VIPAS39 Polyclonal antibody

VIPAS39 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20771-1-AP

Synonyms

C14orf133, hSPE 39, Protein spe 39 homolog, VIPAR, VIPAS39, VPS33B interacting protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VIPAS39 Polyclonal antibody (20771-1-AP)

    WB analysis using 20771-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VIPAS39 Polyclonal antibody (20771-1-AP)

    WB analysis of mouse brain using 20771-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using VIPAS39 Polyclonal antibody (20771-1-AP)

    WB analysis of human kidney using 20771-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VIPAS39 Polyclonal antibody (20771-1-AP)

    IHC staining of mouse liver using 20771-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VIPAS39 Polyclonal antibody (20771-1-AP)

    IHC staining of mouse liver using 20771-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VIPAS39 Polyclonal antibody (20771-1-AP)

    IF Staining of HeLa using 20771-1-AP

    Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VIPAS39 Polyclonal antibody (20771-1-AP)

    IF Staining of HepG2 using 20771-1-AP

    Immunofluorescent analysis of HepG2 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VIPAS39 Polyclonal antibody (20771-1-AP)

    IF Staining of HeLa using 20771-1-AP

    Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VIPAS39 Polyclonal antibody (20771-1-AP)

    IF Staining of HEK-293 using 20771-1-AP

    Immunofluorescent analysis of 293 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, human kidney tissue, HepG2 cells, L02 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20771-1-AP targets VIPAS39 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VIPAS39 fusion protein Ag14762
Full Name chromosome 14 open reading frame 133
Calculated Molecular Weight 493 aa, 57 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC015054
Gene Symbol C14orf133
Gene ID (NCBI) 63894
RRIDAB_10695764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Protocols

Product Specific Protocols
WB protocol for VIPAS39 antibody 20771-1-APDownload protocol
IHC protocol for VIPAS39 antibody 20771-1-APDownload protocol
IF protocol for VIPAS39 antibody 20771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

FERARI is required for Rab11-dependent endocytic recycling.

Authors - Jachen A Solinger
View Article
humanWB

Virus Genes

Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7.

Authors - Ye Li
View Article