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RAB11A/B Polyclonal antibody

RAB11A/B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15903-1-AP

Synonyms

Rab 11, RAB11, RAB11A, RAB11A/B, Ras related protein Rab 11A, YL8



Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HeLa cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15903-1-AP targets RAB11A/B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, monkey, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11A/B fusion protein Ag8742
Full Name RAB11A, member RAS oncogene family
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC013348
Gene symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_2173458
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Protocols

Product Specific Protocols
WB protocol for RAB11A/B antibody 15903-1-APDownload protocol
IHC protocol for RAB11A/B antibody 15903-1-APDownload protocol
IF protocol for RAB11A/B antibody 15903-1-APDownload protocol
IP protocol for RAB11A/B antibody 15903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
canineIF

J Cell Biol

Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion.

Authors - Lee Dolat
humanWB,CoIP

Cell Mol Life Sci

KIF13B mediates VEGFR2 recycling to modulate vascular permeability

Authors - Hyun-Dong Cho
humanIF

Oncogene

Piccolo mediates EGFR signaling and acts as a prognostic biomarker in esophageal squamous cell carcinoma.

Authors - W Zhang
humanWB,IF,CoIP

Clin Transl Med

ANXA6 suppresses the tumorigenesis of cervical cancer through autophagy induction.

Authors - Xin Sun
humanWB

Oncotarget

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

Authors - Hao Bo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by RAB11 polyclonal antibody at dilution of 1:100 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y cell line
FH

Stephen (Verified Customer) (09-12-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours rab11 antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: mouse OC-2 cells
RAB11A/B Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC-2 cells (Cat no:15903-1-AP)