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RAB11A/B Polyclonal antibody
RAB11A/B Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 15903-1-AP
|Positive WB detected in||mouse spleen tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HeLa cells|
|Positive IP detected in||mouse spleen tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15903-1-AP targets RAB11A/B in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||canine, human, monkey, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB11A/B fusion protein Ag8742|
|Full Name||RAB11A, member RAS oncogene family|
|Calculated molecular weight||216 aa, 24 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC013348|
|Gene ID (NCBI)||8766|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).
|Product Specific Protocols|
|WB protocol for RAB11A/B antibody 15903-1-AP||Download protocol|
|IHC protocol for RAB11A/B antibody 15903-1-AP||Download protocol|
|IF protocol for RAB11A/B antibody 15903-1-AP||Download protocol|
|IP protocol for RAB11A/B antibody 15903-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Commun Signal
Zika virus: mapping and reprogramming the entry.
Med Sci Monit
Rab11 Functions as an Oncoprotein via Nuclear Factor kappa B (NF-κB) Signaling Pathway in Human Bladder Carcinoma.
Rab11a Is Overexpressed in Gastric Cancer and Regulates FAK/AKT Signaling.
Dig Dis Sci
Inactivation of MYO5B promotes invasion and motility in gastric cancer cells.
AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.
Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-16-2021)
Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by RAB11 polyclonal antibody at dilution of 1:100 showed strong labelling.
Stephen (Verified Customer) (09-12-2019)
Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours rab11 antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.