RAB11A/B Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB11A/B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

30

Reviews (2)

Cat no : 15903-1-AP

Synonyms

Rab 11, RAB11, RAB11A, RAB11A/B, Ras related protein Rab 11A, YL8

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Validation Data Gallery

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  WB analysis of mouse spleen using 15903-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15903-1-AP

  WB result of RAB11A/B antibody (15903-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A/B transfected HeLa cells.

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  WB analysis of SH-SY5Y using 15903-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/Bantibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15903-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15903-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15903-1-AP (RAB11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 15903-1-AP

  IP result of anti-RAB11A/B (IP:15903-1-AP, 3ug; Detection:15903-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

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  IP experiment of mouse spleen using 15903-1-AP

  IP result of anti-RAB11A/B (IP:15903-1-AP, 4ug; Detection:15903-1-AP 1:1000) with mouse spleen tissue lysate 1920 ug.

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  IHC staining of human heart using 15903-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15903-1-AP (RAB11A/B antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15903-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15903-1-AP (RAB11A/B antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of U2OS using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 15903-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15903-1-AP (RAB11A/B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15903-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RAB11A/B antibody (15903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HeLa cells
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 15 publications below
• CoIP: See 3 publications below

Product Information

15903-1-AP targets RAB11A/B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB11A/B fusion protein Ag8742
• Full Name: RAB11A, member RAS oncogene family
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC013348
• Gene Symbol: RAB11A
• Gene ID (NCBI): 8766
• RRID: AB_2173458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Publications

Species	Application	Title
human	IF	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J
canine	IF	J Cell Biol; Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion.; Authors - Lee Dolat
human	WB,CoIP	Cell Mol Life Sci; KIF13B mediates VEGFR2 recycling to modulate vascular permeability; Authors - Hyun-Dong Cho
human	IF	Oncogene; Piccolo mediates EGFR signaling and acts as a prognostic biomarker in esophageal squamous cell carcinoma.; Authors - W Zhang
human	WB,IF,CoIP	Clin Transl Med; ANXA6 suppresses the tumorigenesis of cervical cancer through autophagy induction.; Authors - Xin Sun
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by RAB11 polyclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: SH-SY5Y cell line

FH

Stephen (Verified Customer) (09-12-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours rab11 antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: mouse OC-2 cells

RAB11A/B Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC-2 cells (Cat no:15903-1-AP)