VPS33A Polyclonal antibody

VPS33A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 16896-1-AP

Synonyms

hVPS33A, VPS33A



Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells, human cerebellum tissue, NIH/3T3 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

16896-1-AP targets VPS33A in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS33A fusion protein Ag10422
Full Name vacuolar protein sorting 33 homolog A (S. cerevisiae)
Calculated molecular weight 596 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC016617
Gene symbol VPS33A
Gene ID (NCBI) 65082
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS33A is a Sec1/Munc18 family member and is a component of the homotypic fusion and protein sorting (HOPS)-tethering complex. The mammalian HOPS complex comprises six subunits (VPS11, VPS16, VPS18, VPS33A, VPS39, and VPS41) (PMID: 23901104). This complex is required for late endosome-lysosome and autophagosome-lysosome fusion (PMID: 24554770). VPS33A plays a role in vesicular transport to the lysosomal compartment.

Protocols

Product Specific Protocols
WB protocol for VPS33A antibody 16896-1-APDownload protocol
IP protocol for VPS33A antibody 16896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

The small GTPase Arl8b regulates assembly of the mammalian HOPS complex to lysosomes.

Authors - Divya Khatter
humanIF

PLoS Pathog

Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication.

Authors - Aastha Sindhwani
humanWB

J Cell Biol

The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.

Authors - Rituraj Marwaha
humanWB

Autophagy

Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Authors - Qiuhong Shen